Expression of adrenomedullin mRNA in the human brain and pituitary

Kazuhiro Takahashi, Fumitoshi Satoh, Masahiko Sone, Osamu Murakami, Hironobu Sasano, Toraichi Mouri, Shigeki Shibahara

Research output: Contribution to journalArticlepeer-review

58 Citations (Scopus)

Abstract

We have recently reported the presence of immunoreactive (IR) adrenomedullin (ADM) in the human brain. In the present study, the expression of ADM mRNA was studied by Northern blot analysis in the human brain and pituitary, and the presence of IR-ADM in the human pituitary was studied by radioimmunoassay. ADM mRNA was clearly detected in every region of the brain examined and in the pituitary. High concentrations of IR-ADM were present in the whole pituitary (16.7 ± 2.0 pmol/g wet weight, mean ± SEM, n = 4). Reverse phase high performance liquid chromatography of the pituitary showed a peak eluting in the position of human ADM(1-52). These findings suggest that ADM acts as a neuromodulator or a neurotransmitter in the brain, and as an autocrine factor, a paracrine factor, or a neurohormone in the pituitary.

Original languageEnglish
Pages (from-to)1051-1053
Number of pages3
JournalPeptides
Volume18
Issue number7
DOIs
Publication statusPublished - 1997

Keywords

  • Adrenomedullin
  • Brain
  • Hypothalamus
  • Northern blot
  • Pituitary
  • RNA
  • Radioimmunoassay

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Cellular and Molecular Neuroscience

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