Expression of adrenomedullin mRNA in the human brain and pituitary

Kazuhiro Takahashi; Fumitoshi Satoh; Masahiko Sone; Osamu Murakami; Hironobu Sasano; Toraichi Mouri; Shigeki Shibahara

doi:10.1016/S0196-9781(97)00038-7

Expression of adrenomedullin mRNA in the human brain and pituitary

Kazuhiro Takahashi, Fumitoshi Satoh, Masahiko Sone, Osamu Murakami, Hironobu Sasano, Toraichi Mouri, Shigeki Shibahara

Research output: Contribution to journal › Article › peer-review

58 Citations (Scopus)

Abstract

We have recently reported the presence of immunoreactive (IR) adrenomedullin (ADM) in the human brain. In the present study, the expression of ADM mRNA was studied by Northern blot analysis in the human brain and pituitary, and the presence of IR-ADM in the human pituitary was studied by radioimmunoassay. ADM mRNA was clearly detected in every region of the brain examined and in the pituitary. High concentrations of IR-ADM were present in the whole pituitary (16.7 ± 2.0 pmol/g wet weight, mean ± SEM, n = 4). Reverse phase high performance liquid chromatography of the pituitary showed a peak eluting in the position of human ADM(1-52). These findings suggest that ADM acts as a neuromodulator or a neurotransmitter in the brain, and as an autocrine factor, a paracrine factor, or a neurohormone in the pituitary.

Original language	English
Pages (from-to)	1051-1053
Number of pages	3
Journal	Peptides
Volume	18
Issue number	7
DOIs	https://doi.org/10.1016/S0196-9781(97)00038-7
Publication status	Published - 1997

Keywords

Adrenomedullin
Brain
Hypothalamus
Northern blot
Pituitary
RNA
Radioimmunoassay

ASJC Scopus subject areas

Biochemistry
Physiology
Endocrinology
Cellular and Molecular Neuroscience

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10.1016/S0196-9781(97)00038-7

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title = "Expression of adrenomedullin mRNA in the human brain and pituitary",

abstract = "We have recently reported the presence of immunoreactive (IR) adrenomedullin (ADM) in the human brain. In the present study, the expression of ADM mRNA was studied by Northern blot analysis in the human brain and pituitary, and the presence of IR-ADM in the human pituitary was studied by radioimmunoassay. ADM mRNA was clearly detected in every region of the brain examined and in the pituitary. High concentrations of IR-ADM were present in the whole pituitary (16.7 ± 2.0 pmol/g wet weight, mean ± SEM, n = 4). Reverse phase high performance liquid chromatography of the pituitary showed a peak eluting in the position of human ADM(1-52). These findings suggest that ADM acts as a neuromodulator or a neurotransmitter in the brain, and as an autocrine factor, a paracrine factor, or a neurohormone in the pituitary.",

keywords = "Adrenomedullin, Brain, Hypothalamus, Northern blot, Pituitary, RNA, Radioimmunoassay",

author = "Kazuhiro Takahashi and Fumitoshi Satoh and Masahiko Sone and Osamu Murakami and Hironobu Sasano and Toraichi Mouri and Shigeki Shibahara",

year = "1997",

doi = "10.1016/S0196-9781(97)00038-7",

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pages = "1051--1053",

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T1 - Expression of adrenomedullin mRNA in the human brain and pituitary

AU - Takahashi, Kazuhiro

AU - Satoh, Fumitoshi

AU - Sone, Masahiko

AU - Murakami, Osamu

AU - Sasano, Hironobu

AU - Mouri, Toraichi

AU - Shibahara, Shigeki

PY - 1997

Y1 - 1997

N2 - We have recently reported the presence of immunoreactive (IR) adrenomedullin (ADM) in the human brain. In the present study, the expression of ADM mRNA was studied by Northern blot analysis in the human brain and pituitary, and the presence of IR-ADM in the human pituitary was studied by radioimmunoassay. ADM mRNA was clearly detected in every region of the brain examined and in the pituitary. High concentrations of IR-ADM were present in the whole pituitary (16.7 ± 2.0 pmol/g wet weight, mean ± SEM, n = 4). Reverse phase high performance liquid chromatography of the pituitary showed a peak eluting in the position of human ADM(1-52). These findings suggest that ADM acts as a neuromodulator or a neurotransmitter in the brain, and as an autocrine factor, a paracrine factor, or a neurohormone in the pituitary.

AB - We have recently reported the presence of immunoreactive (IR) adrenomedullin (ADM) in the human brain. In the present study, the expression of ADM mRNA was studied by Northern blot analysis in the human brain and pituitary, and the presence of IR-ADM in the human pituitary was studied by radioimmunoassay. ADM mRNA was clearly detected in every region of the brain examined and in the pituitary. High concentrations of IR-ADM were present in the whole pituitary (16.7 ± 2.0 pmol/g wet weight, mean ± SEM, n = 4). Reverse phase high performance liquid chromatography of the pituitary showed a peak eluting in the position of human ADM(1-52). These findings suggest that ADM acts as a neuromodulator or a neurotransmitter in the brain, and as an autocrine factor, a paracrine factor, or a neurohormone in the pituitary.

KW - Adrenomedullin

KW - Brain

KW - Hypothalamus

KW - Northern blot

KW - Pituitary

KW - RNA

KW - Radioimmunoassay

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AN - SCOPUS:0030670016

SN - 0196-9781

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SP - 1051

EP - 1053

JO - Peptides

JF - Peptides

IS - 7

ER -

Expression of adrenomedullin mRNA in the human brain and pituitary

Abstract

Keywords

ASJC Scopus subject areas

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