TY - JOUR
T1 - Expression of a DEFICIENS-like gene correlates with the differentiation between sepal and petal in the orchid, Habenaria radiata (Orchidaceae)
AU - Kim, So Young
AU - Yun, Pil Yong
AU - Fukuda, Tatsuya
AU - Ochiai, Toshinori
AU - Yokoyama, Jun
AU - Kameya, Toshiaki
AU - Kanno, Akira
N1 - Funding Information:
We thank Mr. H. Tokairin for collaboration in culturing the plants. We are also grateful to T. Yamashiro, B.-J. Park, T. Ito, T. Nakamura, I.-J. Song, H. Ashizawa, Y. Mashiko, M. Nakada, T. Shishido, N. Kuroiwa, R. Shinohara, M. Komatsu and M. Hirai for providing help and advice. This work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology, Japan.
PY - 2007/2
Y1 - 2007/2
N2 - The B-class MADS-box genes play an important role in controlling petal development in dicots, and orthologs have been isolated from many plants. The Orchidaceae includes many species with petaloid sepals in whorl 1, while there are also several genera with two whorls of perianth, petals and greenish sepals. Since the morphological differentiation between sepals and petals is observed in different orchid groups such as Cypripedium and Habenaria, this characteristic would be evolved independently in Orchidaceae. Habenaria radiata, which is an ornamental plant, has green sepals and white petals in its perianth. Among the cultivars of H. radiata, one floral homeotic mutant has undergone an alteration from sepals to petaloid organs in whorl 1. In order to investigate the molecular mechanism of the morphological differentiation between sepals and petals in Orchidaceae, it is very interesting to analyze the B-class gene expression in wild type and petaloid-sepal mutant of H. radiata. In this study, we isolated and characterized three B-class genes, HrGLO1, HrGLO2 and HrDEF, from this species. Northern hybridization and RT-PCR analyses revealed that HrGLO1 and HrGLO2 were expressed in sepals, petals and columns, whereas HrDEF expression was detected only in the petals and columns, but not in the first whorl, sepals. In the petaloid-sepal mutant, all three B-class genes were expressed in petaloid-sepals, petals and columns. These results suggest that the distinctive expression of HrDEF plays a role in differentiation between sepal and petal in H. radiata.
AB - The B-class MADS-box genes play an important role in controlling petal development in dicots, and orthologs have been isolated from many plants. The Orchidaceae includes many species with petaloid sepals in whorl 1, while there are also several genera with two whorls of perianth, petals and greenish sepals. Since the morphological differentiation between sepals and petals is observed in different orchid groups such as Cypripedium and Habenaria, this characteristic would be evolved independently in Orchidaceae. Habenaria radiata, which is an ornamental plant, has green sepals and white petals in its perianth. Among the cultivars of H. radiata, one floral homeotic mutant has undergone an alteration from sepals to petaloid organs in whorl 1. In order to investigate the molecular mechanism of the morphological differentiation between sepals and petals in Orchidaceae, it is very interesting to analyze the B-class gene expression in wild type and petaloid-sepal mutant of H. radiata. In this study, we isolated and characterized three B-class genes, HrGLO1, HrGLO2 and HrDEF, from this species. Northern hybridization and RT-PCR analyses revealed that HrGLO1 and HrGLO2 were expressed in sepals, petals and columns, whereas HrDEF expression was detected only in the petals and columns, but not in the first whorl, sepals. In the petaloid-sepal mutant, all three B-class genes were expressed in petaloid-sepals, petals and columns. These results suggest that the distinctive expression of HrDEF plays a role in differentiation between sepal and petal in H. radiata.
KW - B-class gene
KW - DEFICIENS-like gene
KW - GLOBOSA-like gene
KW - Habenaria
KW - MADS-box gene
KW - Orchidaceae
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U2 - 10.1016/j.plantsci.2006.09.009
DO - 10.1016/j.plantsci.2006.09.009
M3 - Article
AN - SCOPUS:33846260080
VL - 172
SP - 319
EP - 326
JO - Plant Science
JF - Plant Science
SN - 0168-9452
IS - 2
ER -