TY - JOUR
T1 - Expression of a candidate ataxia-telangiectasia group d gene in cultured fibroblast cell lines and human tissues
AU - Hosoi, Y.
AU - Kapp, L. N.
N1 - Funding Information:
This work was supported by the Office of Health and Environmental Research, US Department of Energy, contract number DE-AC03-76-SF01012, and grants from the A-T Medical Research Foundation and the Thomas Appeal A-T Medical Research Trust .
PY - 1994
Y1 - 1994
N2 - A candidate gene for ataxia-telangiectasia group D (ATDC) has been cloned (Kapp et al. 1992), sequenced and found to be a member of a recently reported gene family (Leonhardt et al. 1994). Transcriptional behaviour of ATDC has been examined in a number of cell lines and human tissues using a 3.0 kb cloned cDNA as a probe. Three normal and two ataxia-telangiectasia (A-T) group D, non-transformed fibroblast cell lines produced a 2.4 kb mRNA transcript. The size of mRNA transcripts seen and the level of expression differ in different human tissues. Many tissues have multiple transcripts of ATDC and the most prominent transcripts observed were 3.0, 2.4 and 1.6 kb. Two out of three SV-40 transformed normal and one SV-40-transformed A-T group D fibroblast cell lines demonstrated no transcription of ATDC by RNA blotting analysis. These results suggest that SV-40 transformation may affect the expression of ATDC. Reverse transcription-polymerase chain reaction analysis showed that ATDC was expressed at low levels in all of these cells. Additional Northern blot analysis demonstrated that X-irradiation with 10 Gy had no effect on ATDC expression at 1, 4 and 24 h after irradiation in either SV-40-transformed normal or in SV-40-transformed A-T group D fibroblast cell lines. Further understanding of ATDC will require cloning of additional transcripts and studies of ATDC protein behaviour.
AB - A candidate gene for ataxia-telangiectasia group D (ATDC) has been cloned (Kapp et al. 1992), sequenced and found to be a member of a recently reported gene family (Leonhardt et al. 1994). Transcriptional behaviour of ATDC has been examined in a number of cell lines and human tissues using a 3.0 kb cloned cDNA as a probe. Three normal and two ataxia-telangiectasia (A-T) group D, non-transformed fibroblast cell lines produced a 2.4 kb mRNA transcript. The size of mRNA transcripts seen and the level of expression differ in different human tissues. Many tissues have multiple transcripts of ATDC and the most prominent transcripts observed were 3.0, 2.4 and 1.6 kb. Two out of three SV-40 transformed normal and one SV-40-transformed A-T group D fibroblast cell lines demonstrated no transcription of ATDC by RNA blotting analysis. These results suggest that SV-40 transformation may affect the expression of ATDC. Reverse transcription-polymerase chain reaction analysis showed that ATDC was expressed at low levels in all of these cells. Additional Northern blot analysis demonstrated that X-irradiation with 10 Gy had no effect on ATDC expression at 1, 4 and 24 h after irradiation in either SV-40-transformed normal or in SV-40-transformed A-T group D fibroblast cell lines. Further understanding of ATDC will require cloning of additional transcripts and studies of ATDC protein behaviour.
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U2 - 10.1080/09553009414551891
DO - 10.1080/09553009414551891
M3 - Article
C2 - 7530755
AN - SCOPUS:0028585984
VL - 66
SP - S71-S76
JO - International Journal of Radiation Biology
JF - International Journal of Radiation Biology
SN - 0955-3002
IS - 6
ER -