TY - JOUR
T1 - Expression of 11β-hydroxysteroid dehydrogenase type 2 (11βHSD-2) in the developing human adrenal gland and human adrenal cortical carcinoma and adenoma
AU - Coulter, Catherine L.
AU - Smith, Robin E.
AU - Stowasser, Michael
AU - Sasano, Hironobu
AU - Krozowski, Zygmunt S.
AU - Gordon, Richard D.
N1 - Funding Information:
This work was supported by the National health and Medical Research Council of Australia by a C.J. Martin Fellowship to Dr Catherine L. Coulter and a Block grant to the Baker Medical Research Institute and by the National Heart Foundation of Australia by a Clinical Research Fellowship to Dr Michael Stowasser. The authors acknowledge the assistance of Dr David Cohn (Queensland Medical Laboratory) for preparation of pathological adrenal tissue specimens and Dr Robert Jaffe (University of California, San Francisco) for assistance in obtaining the human foetal and postnatal adrenal specimens. We thank Drs Kevin Li and Rod Dilley (Baker Medical Research Institute) for assistance with the western blot analysis and photomicrography, respectively.
PY - 1999/8/20
Y1 - 1999/8/20
N2 - The aim of this study was to investigate the ontogeny of localization of 11βHSD-2 protein in the human adrenal gland. In addition, we have investigated the effects of abnormal adrenal function on 11βHSD-2 by determining the pattern of localization of 11βHSD-2 protein, and the amount and level of expression of 11βHSD-2 mRNA and protein in human adrenal cortical carcinoma and adenoma. In the human foetal adrenal gland 11βHSD-2 immunoreactivity (11βHSD-2-ir) was detected in the foetal zone, whereas in normal adult adrenal glands 11βHSD-2-ir was not detected by immunocytochemistry. In adrenal cortical carcinoma and adenoma, 11βHSD-2-ir was detectable in specific regions, which have been identified as steroid synthesizing cells using 3βHSD-ir as a marker. In adrenal cortical carcinoma and adenoma, 11βHSD-2 mRNA and 11βHSD-2 protein were detected by nuclease protection analysis and by western blot analysis, respectively. In summary, 11βHSD-2-ir was detected in the foetal zone of the mid-gestation human foetal adrenal, whereas, 11βHSD-2-ir was not detectable in the postnatal or normal adult adrenal gland. 11βHSD-2 protein and mRNA was induced in adult human adrenal cortical carcinoma and adenoma. The induction of expression of 11βHSD-2 in the adrenal cortex suggests a possible role in regulating abnormal adrenal steroidogenic function in these patients. Copyright (C) 1999 Elsevier Science Ireland Ltd.
AB - The aim of this study was to investigate the ontogeny of localization of 11βHSD-2 protein in the human adrenal gland. In addition, we have investigated the effects of abnormal adrenal function on 11βHSD-2 by determining the pattern of localization of 11βHSD-2 protein, and the amount and level of expression of 11βHSD-2 mRNA and protein in human adrenal cortical carcinoma and adenoma. In the human foetal adrenal gland 11βHSD-2 immunoreactivity (11βHSD-2-ir) was detected in the foetal zone, whereas in normal adult adrenal glands 11βHSD-2-ir was not detected by immunocytochemistry. In adrenal cortical carcinoma and adenoma, 11βHSD-2-ir was detectable in specific regions, which have been identified as steroid synthesizing cells using 3βHSD-ir as a marker. In adrenal cortical carcinoma and adenoma, 11βHSD-2 mRNA and 11βHSD-2 protein were detected by nuclease protection analysis and by western blot analysis, respectively. In summary, 11βHSD-2-ir was detected in the foetal zone of the mid-gestation human foetal adrenal, whereas, 11βHSD-2-ir was not detectable in the postnatal or normal adult adrenal gland. 11βHSD-2 protein and mRNA was induced in adult human adrenal cortical carcinoma and adenoma. The induction of expression of 11βHSD-2 in the adrenal cortex suggests a possible role in regulating abnormal adrenal steroidogenic function in these patients. Copyright (C) 1999 Elsevier Science Ireland Ltd.
KW - Cortisol
KW - Metabolism
KW - Steroids
KW - Synthesis
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U2 - 10.1016/S0303-7207(99)00077-5
DO - 10.1016/S0303-7207(99)00077-5
M3 - Article
C2 - 10509802
AN - SCOPUS:0032589357
VL - 154
SP - 71
EP - 77
JO - Molecular and Cellular Endocrinology
JF - Molecular and Cellular Endocrinology
SN - 0303-7207
IS - 1-2
ER -