Expression of γ-hemolysin regulated by sae in Staphylococcus aureus strain Smith 5R

Kazuko Yamazaki, Fuminori Kato, Yoshiyuki Kamio, Jun Kaneko

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

Staphylococcus aureus strain Smith 5R produces a two-component pore-forming toxin and forms a rough-surfaced colony with hemolytic haloes on human red blood cell plates (R[+]). Serial subcultures of the strain in broth caused the appearance of γ-hemolysin negative variants with a smooth colony shape (S[-]), and the S[-] valiant became predominant in culture. The R[+] strain, in which agrA is naturally disrupted by an insertion of IS1181, produced high levels of γ-hemolysin. In the S[-] variant, expression of both hlg and lukS-F mRNAs was strongly reduced. Nucleotide sequencing of the sae locus revealed that all isolated S[-] variants had spontaneous mutations in the sae locus. Recovery of γ-hemolysin productivity in S[-] by transformation of the wild-type sae allele strongly suggested that the expression of γ-hemolysin is positively regulated by sae in an agr-independent manner.

Original languageEnglish
Pages (from-to)174-180
Number of pages7
JournalFEMS Microbiology Letters
Volume259
Issue number2
DOIs
Publication statusPublished - 2006 Jun

Keywords

  • Regulation
  • Staphylococcus aureus
  • sae
  • γ-hemolysin expression

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

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