Expression of α-tocopherol-associated genes and α-tocopherol accumulation in Japanese black (Wagyu) calves with and without α-tocopherol supplementation

S. Haga, M. Nakano, H. Ishizaki, S. G. Roh, K. Katoh

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

The aim of the study was to clarify 1) the distribution of 6 α-tocopherol (α-Toc)-associated gene expressions in 20 major tissues, including metabolic, reproductive, endocrine, immune, and digestive and absorptive tissues, in relation to α-Toc status and 2) the change in expression patterns of the genes induced when α-Toc was orally administered to Japanese Black (JB) calves. This study examined weaned male JB calves (n = 10), of which 5 calves were orally administered α-Toc for 2 wk (30 IU∙kg-1 BW∙d-1; TOC group). The others did not receive the α-Toc supplement and were the control (CONT) group. The 20 tissues and venous blood (serum) were sampled on the final day. In both groups, the mean mRNA expression levels for α-Toc transfer protein, afamin (AFM), ATP-binding cassette transporter A1, and tocopherol-associated protein were greatest in the liver (P < 0.05), whereas scavenger receptor class B, Type I (SR-BI) mRNA was greatest in the adrenal gland (P < 0.05). The gene for cytochrome P450 family 4, subfamily F, polypeptide 2 was most highly expressed in the liver, testes, and adrenal gland. The α-Toc content was greatest (P < 0.05) in the testes of the 20 sampled tissues in the CONT group. However, the levels in the testes and jejunum were similar and greater (P < 0.05) than the levels in the other 18 tissues in the TOC group. The mean increase in α-Toc levels after oral α-Toc administration (mean α-Toc content for the TOC group divided by the CONT group content) were greater (P < 0.05) in the jejunum (40.7-fold) and duodenum and liver (26.3-and 23.1-fold) than in the serum (7.8-fold). In the liver, α-Toc administration significantly increased (P < 0.05) the AFM and SR-BI mRNA expression levels. The results show that the liver may play an important role in the regulation of α-Toc disposition, but other peripheral tissues that accumulate large amounts of α-Toc could moderate the local α-Toc status and functions, as inferred from the high expressions of the α-Toc-associated genes in JB calves.

Original languageEnglish
Pages (from-to)4048-4057
Number of pages10
JournalJournal of animal science
Volume93
Issue number8
DOIs
Publication statusPublished - 2015 Aug 6
Externally publishedYes

Keywords

  • Cattle
  • Vitamin E
  • mRNA expression
  • α-tocopherol binding protein

ASJC Scopus subject areas

  • Food Science
  • Animal Science and Zoology
  • Genetics

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