TY - JOUR
T1 - Expression of α-Amylase Isozymes in Human Thyroid Tissues
AU - Doi, Sadayuki
AU - Tornita, Naohiro
AU - Yokouchi, Hideoki
AU - Horii, Akira
AU - Yasuda, Tadashi
AU - Matsubara, Kenichi
AU - Kobayashi, Tetsuro
AU - Takai, Sinichiro
AU - Mori, Takesada
N1 - Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.
PY - 1991/7
Y1 - 1991/7
N2 - Expression of a-amylase genes in thyroid tissues was studied by assaying the total amylase activity as well as by using immunohistochemical and Northern blot analysis. The amylase genes expressed were determined by a combination of the polymerase chain reaction (PCR) and blot analysis using synthetic probes specific for the three known amylase isozyme complementary DNAs. The samples consisted of tissues from 18 human thyroid carcinomas (11 well-differentiated carcinomas, 2 poorly differentiated carcinomas, 1 anaplastic carcinoma, and 4 medullary carcinomas) and 9 specimens of nonmalignant thyroid tissue (2 were from nontumorous regions of resected glands and 7 were thyroid tissue from a patient with Graves’ disease). Salivary-type amylase was expressed at a relatively high level in nonmalignant thyroid tissue and well-differentiated carcinoma and could be detected by Northern blot analysis. In poorly differentiated carcinoma, it was detected only by the PCR, while in anaplastic or medullary carcinoma, it was not detected even by the PCR. Thus, the expression of salivary-type amylase was characteristic of welldifferentiated follicular cells. These observations suggest that salivarytype amylase expression may be a marker for identifying the histogenesis and stage of differentiation of thyroid cancer. In addition, the AMY2B gene product was detected in all different types of cells examined, although its expression was only detectable by the PCR. Pancreatic type amylase was not detected in any of the samples.
AB - Expression of a-amylase genes in thyroid tissues was studied by assaying the total amylase activity as well as by using immunohistochemical and Northern blot analysis. The amylase genes expressed were determined by a combination of the polymerase chain reaction (PCR) and blot analysis using synthetic probes specific for the three known amylase isozyme complementary DNAs. The samples consisted of tissues from 18 human thyroid carcinomas (11 well-differentiated carcinomas, 2 poorly differentiated carcinomas, 1 anaplastic carcinoma, and 4 medullary carcinomas) and 9 specimens of nonmalignant thyroid tissue (2 were from nontumorous regions of resected glands and 7 were thyroid tissue from a patient with Graves’ disease). Salivary-type amylase was expressed at a relatively high level in nonmalignant thyroid tissue and well-differentiated carcinoma and could be detected by Northern blot analysis. In poorly differentiated carcinoma, it was detected only by the PCR, while in anaplastic or medullary carcinoma, it was not detected even by the PCR. Thus, the expression of salivary-type amylase was characteristic of welldifferentiated follicular cells. These observations suggest that salivarytype amylase expression may be a marker for identifying the histogenesis and stage of differentiation of thyroid cancer. In addition, the AMY2B gene product was detected in all different types of cells examined, although its expression was only detectable by the PCR. Pancreatic type amylase was not detected in any of the samples.
UR - http://www.scopus.com/inward/record.url?scp=0026051466&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026051466&partnerID=8YFLogxK
M3 - Article
C2 - 2054791
AN - SCOPUS:0026051466
VL - 51
SP - 3544
EP - 3549
JO - Cancer Research
JF - Cancer Research
SN - 0008-5472
IS - 13
ER -