Expression of α-1,3 linkage-containing oligomannosyl residues in a cell-wall mannan of Candida tropicalis grown in yeast extract-Sabouraud liquid medium under acidic conditions

Hidemitsu Kobayashi; Nobuyuki Shibata; Akifumi Suzuki; Shin ichi Takahashi; Masako Suzuki; Kyoko Matsuda; Kanehiko Hisamichi; Shigeo Suzuki

doi:10.1016/0014-5793(94)80576-8

Expression of α-1,3 linkage-containing oligomannosyl residues in a cell-wall mannan of Candida tropicalis grown in yeast extract-Sabouraud liquid medium under acidic conditions

Hidemitsu Kobayashi, Nobuyuki Shibata, Akifumi Suzuki, Shin ichi Takahashi, Masako Suzuki, Kyoko Matsuda, Kanehiko Hisamichi, Shigeo Suzuki

Clinical Research, Innovation and Education Center

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Abstract

We investigated the cell-wall mannan obtained from Candida tropicalis IFO 1647 strain cells grown in yeast extract-Sabouraud medium at pH 3.0 by two-dimensional homonuclear Hartmann-Hahn spectroscopy. The results indicate that the phosphate group and the side chains containing a β-1,2-linked mannopyranose unit decreased compared to those of mannan from cells grown under conventional conditions (pH 5.9) with concomitant expression of α-1,3 linkage-containing oligomannosyl side chains. The results of acetolysis of these mannans indicated that the presence of α-1,3-linked mannopyranose unit existed in side chains corresponding to pentaose and hexaose, Manpα1-3Manpα1-2Manpα1-2Manpα1-2Man, and Manpα1-2Manpα1-3Manpα1-2Manpα1-2Manpα1-2Man, in the mannan from cells grown at pH 3.0.

Original language	English
Pages (from-to)	19-22
Number of pages	4
Journal	FEBS Letters
Volume	342
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(94)80576-8
Publication status	Published - 1994 Mar 28

Keywords

Candida tropicalis
Chemical structure
Nuclear magnetic resonance
Yeast cell-wall mannan
α-1,3 Linkage
β-1,2 Linkage

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(94)80576-8

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Kobayashi, H., Shibata, N., Suzuki, A., Takahashi, S. I., Suzuki, M., Matsuda, K., Hisamichi, K., & Suzuki, S. (1994). Expression of α-1,3 linkage-containing oligomannosyl residues in a cell-wall mannan of Candida tropicalis grown in yeast extract-Sabouraud liquid medium under acidic conditions. FEBS Letters, 342(1), 19-22. https://doi.org/10.1016/0014-5793(94)80576-8

Kobayashi, H, Shibata, N, Suzuki, A, Takahashi, SI, Suzuki, M, Matsuda, K, Hisamichi, K & Suzuki, S 1994, 'Expression of α-1,3 linkage-containing oligomannosyl residues in a cell-wall mannan of Candida tropicalis grown in yeast extract-Sabouraud liquid medium under acidic conditions', FEBS Letters, vol. 342, no. 1, pp. 19-22. https://doi.org/10.1016/0014-5793(94)80576-8

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abstract = "We investigated the cell-wall mannan obtained from Candida tropicalis IFO 1647 strain cells grown in yeast extract-Sabouraud medium at pH 3.0 by two-dimensional homonuclear Hartmann-Hahn spectroscopy. The results indicate that the phosphate group and the side chains containing a β-1,2-linked mannopyranose unit decreased compared to those of mannan from cells grown under conventional conditions (pH 5.9) with concomitant expression of α-1,3 linkage-containing oligomannosyl side chains. The results of acetolysis of these mannans indicated that the presence of α-1,3-linked mannopyranose unit existed in side chains corresponding to pentaose and hexaose, Manpα1-3Manpα1-2Manpα1-2Manpα1-2Man, and Manpα1-2Manpα1-3Manpα1-2Manpα1-2Manpα1-2Man, in the mannan from cells grown at pH 3.0.",

keywords = "Candida tropicalis, Chemical structure, Nuclear magnetic resonance, Yeast cell-wall mannan, α-1,3 Linkage, β-1,2 Linkage",

author = "Hidemitsu Kobayashi and Nobuyuki Shibata and Akifumi Suzuki and Takahashi, {Shin ichi} and Masako Suzuki and Kyoko Matsuda and Kanehiko Hisamichi and Shigeo Suzuki",

year = "1994",

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doi = "10.1016/0014-5793(94)80576-8",

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AU - Kobayashi, Hidemitsu

AU - Shibata, Nobuyuki

AU - Suzuki, Akifumi

AU - Takahashi, Shin ichi

AU - Suzuki, Masako

AU - Matsuda, Kyoko

AU - Hisamichi, Kanehiko

AU - Suzuki, Shigeo

PY - 1994/3/28

Y1 - 1994/3/28

N2 - We investigated the cell-wall mannan obtained from Candida tropicalis IFO 1647 strain cells grown in yeast extract-Sabouraud medium at pH 3.0 by two-dimensional homonuclear Hartmann-Hahn spectroscopy. The results indicate that the phosphate group and the side chains containing a β-1,2-linked mannopyranose unit decreased compared to those of mannan from cells grown under conventional conditions (pH 5.9) with concomitant expression of α-1,3 linkage-containing oligomannosyl side chains. The results of acetolysis of these mannans indicated that the presence of α-1,3-linked mannopyranose unit existed in side chains corresponding to pentaose and hexaose, Manpα1-3Manpα1-2Manpα1-2Manpα1-2Man, and Manpα1-2Manpα1-3Manpα1-2Manpα1-2Manpα1-2Man, in the mannan from cells grown at pH 3.0.

AB - We investigated the cell-wall mannan obtained from Candida tropicalis IFO 1647 strain cells grown in yeast extract-Sabouraud medium at pH 3.0 by two-dimensional homonuclear Hartmann-Hahn spectroscopy. The results indicate that the phosphate group and the side chains containing a β-1,2-linked mannopyranose unit decreased compared to those of mannan from cells grown under conventional conditions (pH 5.9) with concomitant expression of α-1,3 linkage-containing oligomannosyl side chains. The results of acetolysis of these mannans indicated that the presence of α-1,3-linked mannopyranose unit existed in side chains corresponding to pentaose and hexaose, Manpα1-3Manpα1-2Manpα1-2Manpα1-2Man, and Manpα1-2Manpα1-3Manpα1-2Manpα1-2Manpα1-2Man, in the mannan from cells grown at pH 3.0.

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KW - Chemical structure

KW - Nuclear magnetic resonance

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KW - α-1,3 Linkage

KW - β-1,2 Linkage

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Expression of α-1,3 linkage-containing oligomannosyl residues in a cell-wall mannan of Candida tropicalis grown in yeast extract-Sabouraud liquid medium under acidic conditions

Abstract

Keywords

ASJC Scopus subject areas

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