Expression of α-1,3 linkage-containing oligomannosyl residues in a cell-wall mannan of Candida tropicalis grown in yeast extract-Sabouraud liquid medium under acidic conditions

Hidemitsu Kobayashi, Nobuyuki Shibata, Akifumi Suzuki, Shin ichi Takahashi, Masako Suzuki, Kyoko Matsuda, Kanehiko Hisamichi, Shigeo Suzuki

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9 Citations (Scopus)

Abstract

We investigated the cell-wall mannan obtained from Candida tropicalis IFO 1647 strain cells grown in yeast extract-Sabouraud medium at pH 3.0 by two-dimensional homonuclear Hartmann-Hahn spectroscopy. The results indicate that the phosphate group and the side chains containing a β-1,2-linked mannopyranose unit decreased compared to those of mannan from cells grown under conventional conditions (pH 5.9) with concomitant expression of α-1,3 linkage-containing oligomannosyl side chains. The results of acetolysis of these mannans indicated that the presence of α-1,3-linked mannopyranose unit existed in side chains corresponding to pentaose and hexaose, Manpα1-3Manpα1-2Manpα1-2Manpα1-2Man, and Manpα1-2Manpα1-3Manpα1-2Manpα1-2Manpα1-2Man, in the mannan from cells grown at pH 3.0.

Original languageEnglish
Pages (from-to)19-22
Number of pages4
JournalFEBS Letters
Volume342
Issue number1
DOIs
Publication statusPublished - 1994 Mar 28
Externally publishedYes

Keywords

  • Candida tropicalis
  • Chemical structure
  • Nuclear magnetic resonance
  • Yeast cell-wall mannan
  • α-1,3 Linkage
  • β-1,2 Linkage

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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