Expression and transport of candida tropicalis peroxisomal acyl-coenzyme a oxidase in the yeast candida maltosa

Tatsuyuki Kamiryo, Yuji Sakasegawa, Hironobu Tan

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

The genes POX2 and POX4, which encode the subunits (PXP-2 and PXP-4) of peroxisomal fatty acyl-coenzyme A oxidase of Candida tropicalis, were introduced into the related yeast Candida maltosa. The cells transformed with POX2 or POX4 gave much PXP-2 or PXP-4 in the purified peroxisomes. The polypeptides associated with the heterologous organelle were resistant to added protease, implying that they were transported into the peroxisomes. Genes for curtailed versions of PXP-4 were constructed in vitro and introduced into the host cells. Peptide-C, the COOH-terminal two-thirds of PXP-4, was efficiently transported into the host peroxisomes, and the polypeptide containing the NH2-terminal one-third was also, in much lesser amount. These and other results suggested that there were at least two regions of peroxisomal targeting information in PXP-4 and the primary information was internal. The deletions in Peptide-C inhibited the transport of many, but not all, of the host-cell peroxisomal polypeptides. This suggested heterogeneous transport systems on the peroxisomal membrane.

Original languageEnglish
Pages (from-to)179-186
Number of pages8
JournalAgricultural and Biological Chemistry
Volume53
Issue number1
DOIs
Publication statusPublished - 1989 Jan

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

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