Expression and sub-cellular localization of human ABH family molecules

Kazutake Tsujikawa, Kazuhisa Koike, Kaori Kitae, Aina Shinkawa, Hiroshi Arima, Takashi Suzuki, Mutsumi Tsuchiya, Yasutaka Makino, Tatsuhiko Furukawa, Noboru Konishi, Hiroshi Yamamoto

Research output: Contribution to journalArticlepeer-review

64 Citations (Scopus)

Abstract

AlkB is an Escherichia coli protein that catalyses the oxidative demethylation of 1-methyladenine and 3-methylcytosine in DNA and RNA. The enzyme activity of AlkB is dependent on a 2-oxoglutarate- and Fe(II)-dependent (2OG-Fe[II]) oxygenase domain. Human AlkB homologues (hABH), hABH1, hABH2 and hABH3, which also possess the 2OG-Fe(II) oxygenase domain, have previously been identified. Recent bioinformatics analysis suggests the existence of an additional five ABH genes in humans. In this study, we identified the hABH4-hABH7 mRNAs and determined their expression in human tissues. Moreover, an hABH2 splice variant lacking the 2OG-Fe(II) oxygenase domain and a new gene, hABH8, were cloned from testis cDNA. hABH8 possesses not only the 2OG-Fe(II) oxygenase domain but both an RNA-binding motif and a methyl-transferase domain. mRNA of the eight hABH molecules was detected in the 16 normal human tissues examined. The sub-cellular localization of EmGFP-hABH8 was restricted to the cytoplasm. EmGFP-hABH1, 3, 4, 6 and 7 were localized in both the cytoplasm and nuclei. Interestingly, the EmGFP-hABH2 splice variant localized in nucleoplasm with a dot-like pattern. In some HeLa cells transfected with EmGFP-hABH5, dot-like fluorescence was also detected in the cytoplasm. These observations provide important information for the future annotation of the hABH family of molecules.

Original languageEnglish
Pages (from-to)1105-1116
Number of pages12
JournalJournal of Cellular and Molecular Medicine
Volume11
Issue number5
DOIs
Publication statusPublished - 2007 Sep

Keywords

  • 2-oxoglutarate-Fe(II) oxygenase domain
  • AlkB
  • DNA repair
  • Demethylation

ASJC Scopus subject areas

  • Molecular Medicine
  • Cell Biology

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