Expression and intracellular localization of mouse Vasa-homologue protein during germ cell development

Yayoi Toyooka; Naoki Tsunekawa; Yoshihiko Takahashi; Yasuhisa Matsui; Michio Satoh; Toshiaki Noce

doi:10.1016/S0925-4773(00)00283-5

Expression and intracellular localization of mouse Vasa-homologue protein during germ cell development

Yayoi Toyooka, Naoki Tsunekawa, Yoshihiko Takahashi, Yasuhisa Matsui, Michio Satoh, Toshiaki Noce

Cell Resource Center for Biomedical Research

Research output: Contribution to journal › Article › peer-review

414 Citations (Scopus)

Abstract

To demonstrate the cellular and subcellular localization of mouse vasa homologue protein during germ cell development, specific antibody was raised against the full-length MVH protein. The immunohistochemical analyses demonstrated that MVH protein was exclusively expressed in primordial germ cells just after their colonization of embryonic gonads and in germ cells undergoing gametogenic processes until the post-meiotic stage in both males and females. The co-culture of EG cells with gonadal somatic cells indicated inductive MVH expression caused by an intercellular interaction with gonadal somatic cells. In adult testis, MVH protein was localized in the cytoplasm of spermatogenic cells, including chromatoid bodies in spermatids, known to be a perinuclear nuage structure which includes polar granules that contain VASA protein in Drosophila. Copyright (C) 2000 Elsevier Science Ireland Ltd.

Original language	English
Pages (from-to)	139-149
Number of pages	11
Journal	Mechanisms of Development
Volume	93
Issue number	1-2
DOIs	https://doi.org/10.1016/S0925-4773(00)00283-5
Publication status	Published - 2000 May 1

Keywords

4C9
Cattle
Chromatoid body
Drosophila
EG cell
ES cell
Flying fox
Follicle
Genital ridge
Germ plasm
Gonad
Gonadal somatic cell
Horse
Human
Immuno-electron microscopy
Immunocytochemistry
Mammal
Matri-gel
Monkey
Mouse
Oocyte
Oogenesis
Ovary
Pig
Polar granule
Primordial germ cell
RNA helicase
SSEA-1
Spermatid
Spermatocyte
Spermatogenesis
Supporting cell
Testis
Totipotency
Translational regulation
Vasa

ASJC Scopus subject areas

Embryology
Developmental Biology

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title = "Expression and intracellular localization of mouse Vasa-homologue protein during germ cell development",

abstract = "To demonstrate the cellular and subcellular localization of mouse vasa homologue protein during germ cell development, specific antibody was raised against the full-length MVH protein. The immunohistochemical analyses demonstrated that MVH protein was exclusively expressed in primordial germ cells just after their colonization of embryonic gonads and in germ cells undergoing gametogenic processes until the post-meiotic stage in both males and females. The co-culture of EG cells with gonadal somatic cells indicated inductive MVH expression caused by an intercellular interaction with gonadal somatic cells. In adult testis, MVH protein was localized in the cytoplasm of spermatogenic cells, including chromatoid bodies in spermatids, known to be a perinuclear nuage structure which includes polar granules that contain VASA protein in Drosophila. Copyright (C) 2000 Elsevier Science Ireland Ltd.",

keywords = "4C9, Cattle, Chromatoid body, Drosophila, EG cell, ES cell, Flying fox, Follicle, Genital ridge, Germ plasm, Gonad, Gonadal somatic cell, Horse, Human, Immuno-electron microscopy, Immunocytochemistry, Mammal, Matri-gel, Monkey, Mouse, Oocyte, Oogenesis, Ovary, Pig, Polar granule, Primordial germ cell, RNA helicase, SSEA-1, Spermatid, Spermatocyte, Spermatogenesis, Supporting cell, Testis, Totipotency, Translational regulation, Vasa",

author = "Yayoi Toyooka and Naoki Tsunekawa and Yoshihiko Takahashi and Yasuhisa Matsui and Michio Satoh and Toshiaki Noce",

note = "Funding Information: The authors thank Drs. Yukiko Yamazaki, Miho Noguchi and Ryuko Akasu for their technical assistance, and Dr. Satomi Tanaka for helpful suggestions on this work. This work was supported in part by a Grant-in-aid from the Ministry of Education, Science and Culture, Japan.",

year = "2000",

month = may,

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doi = "10.1016/S0925-4773(00)00283-5",

language = "English",

volume = "93",

pages = "139--149",

journal = "Mechanisms of Development",

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T1 - Expression and intracellular localization of mouse Vasa-homologue protein during germ cell development

AU - Toyooka, Yayoi

AU - Tsunekawa, Naoki

AU - Takahashi, Yoshihiko

AU - Matsui, Yasuhisa

AU - Satoh, Michio

AU - Noce, Toshiaki

N1 - Funding Information: The authors thank Drs. Yukiko Yamazaki, Miho Noguchi and Ryuko Akasu for their technical assistance, and Dr. Satomi Tanaka for helpful suggestions on this work. This work was supported in part by a Grant-in-aid from the Ministry of Education, Science and Culture, Japan.

PY - 2000/5/1

Y1 - 2000/5/1

N2 - To demonstrate the cellular and subcellular localization of mouse vasa homologue protein during germ cell development, specific antibody was raised against the full-length MVH protein. The immunohistochemical analyses demonstrated that MVH protein was exclusively expressed in primordial germ cells just after their colonization of embryonic gonads and in germ cells undergoing gametogenic processes until the post-meiotic stage in both males and females. The co-culture of EG cells with gonadal somatic cells indicated inductive MVH expression caused by an intercellular interaction with gonadal somatic cells. In adult testis, MVH protein was localized in the cytoplasm of spermatogenic cells, including chromatoid bodies in spermatids, known to be a perinuclear nuage structure which includes polar granules that contain VASA protein in Drosophila. Copyright (C) 2000 Elsevier Science Ireland Ltd.

AB - To demonstrate the cellular and subcellular localization of mouse vasa homologue protein during germ cell development, specific antibody was raised against the full-length MVH protein. The immunohistochemical analyses demonstrated that MVH protein was exclusively expressed in primordial germ cells just after their colonization of embryonic gonads and in germ cells undergoing gametogenic processes until the post-meiotic stage in both males and females. The co-culture of EG cells with gonadal somatic cells indicated inductive MVH expression caused by an intercellular interaction with gonadal somatic cells. In adult testis, MVH protein was localized in the cytoplasm of spermatogenic cells, including chromatoid bodies in spermatids, known to be a perinuclear nuage structure which includes polar granules that contain VASA protein in Drosophila. Copyright (C) 2000 Elsevier Science Ireland Ltd.

KW - 4C9

KW - Cattle

KW - Chromatoid body

KW - Drosophila

KW - EG cell

KW - ES cell

KW - Flying fox

KW - Follicle

KW - Genital ridge

KW - Germ plasm

KW - Gonad

KW - Gonadal somatic cell

KW - Horse

KW - Human

KW - Immuno-electron microscopy

KW - Immunocytochemistry

KW - Mammal

KW - Matri-gel

KW - Monkey

KW - Mouse

KW - Oocyte

KW - Oogenesis

KW - Ovary

KW - Pig

KW - Polar granule

KW - Primordial germ cell

KW - RNA helicase

KW - SSEA-1

KW - Spermatid

KW - Spermatocyte

KW - Spermatogenesis

KW - Supporting cell

KW - Testis

KW - Totipotency

KW - Translational regulation

KW - Vasa

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U2 - 10.1016/S0925-4773(00)00283-5

DO - 10.1016/S0925-4773(00)00283-5

M3 - Article

C2 - 10781947

AN - SCOPUS:0034191987

VL - 93

SP - 139

EP - 149

JO - Mechanisms of Development

JF - Mechanisms of Development

SN - 0925-4773

IS - 1-2

ER -