Abstract
To demonstrate the cellular and subcellular localization of mouse vasa homologue protein during germ cell development, specific antibody was raised against the full-length MVH protein. The immunohistochemical analyses demonstrated that MVH protein was exclusively expressed in primordial germ cells just after their colonization of embryonic gonads and in germ cells undergoing gametogenic processes until the post-meiotic stage in both males and females. The co-culture of EG cells with gonadal somatic cells indicated inductive MVH expression caused by an intercellular interaction with gonadal somatic cells. In adult testis, MVH protein was localized in the cytoplasm of spermatogenic cells, including chromatoid bodies in spermatids, known to be a perinuclear nuage structure which includes polar granules that contain VASA protein in Drosophila. Copyright (C) 2000 Elsevier Science Ireland Ltd.
Original language | English |
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Pages (from-to) | 139-149 |
Number of pages | 11 |
Journal | Mechanisms of Development |
Volume | 93 |
Issue number | 1-2 |
DOIs | |
Publication status | Published - 2000 May 1 |
Externally published | Yes |
Keywords
- 4C9
- Cattle
- Chromatoid body
- Drosophila
- EG cell
- ES cell
- Flying fox
- Follicle
- Genital ridge
- Germ plasm
- Gonad
- Gonadal somatic cell
- Horse
- Human
- Immuno-electron microscopy
- Immunocytochemistry
- Mammal
- Matri-gel
- Monkey
- Mouse
- Oocyte
- Oogenesis
- Ovary
- Pig
- Polar granule
- Primordial germ cell
- RNA helicase
- SSEA-1
- Spermatid
- Spermatocyte
- Spermatogenesis
- Supporting cell
- Testis
- Totipotency
- Translational regulation
- Vasa
ASJC Scopus subject areas
- Embryology
- Developmental Biology