Expression and intracellular localization of mouse Vasa-homologue protein during germ cell development

Yayoi Toyooka, Naoki Tsunekawa, Yoshihiko Takahashi, Yasuhisa Matsui, Michio Satoh, Toshiaki Noce

Research output: Contribution to journalArticlepeer-review

434 Citations (Scopus)

Abstract

To demonstrate the cellular and subcellular localization of mouse vasa homologue protein during germ cell development, specific antibody was raised against the full-length MVH protein. The immunohistochemical analyses demonstrated that MVH protein was exclusively expressed in primordial germ cells just after their colonization of embryonic gonads and in germ cells undergoing gametogenic processes until the post-meiotic stage in both males and females. The co-culture of EG cells with gonadal somatic cells indicated inductive MVH expression caused by an intercellular interaction with gonadal somatic cells. In adult testis, MVH protein was localized in the cytoplasm of spermatogenic cells, including chromatoid bodies in spermatids, known to be a perinuclear nuage structure which includes polar granules that contain VASA protein in Drosophila. Copyright (C) 2000 Elsevier Science Ireland Ltd.

Original languageEnglish
Pages (from-to)139-149
Number of pages11
JournalMechanisms of Development
Volume93
Issue number1-2
DOIs
Publication statusPublished - 2000 May 1
Externally publishedYes

Keywords

  • 4C9
  • Cattle
  • Chromatoid body
  • Drosophila
  • EG cell
  • ES cell
  • Flying fox
  • Follicle
  • Genital ridge
  • Germ plasm
  • Gonad
  • Gonadal somatic cell
  • Horse
  • Human
  • Immuno-electron microscopy
  • Immunocytochemistry
  • Mammal
  • Matri-gel
  • Monkey
  • Mouse
  • Oocyte
  • Oogenesis
  • Ovary
  • Pig
  • Polar granule
  • Primordial germ cell
  • RNA helicase
  • SSEA-1
  • Spermatid
  • Spermatocyte
  • Spermatogenesis
  • Supporting cell
  • Testis
  • Totipotency
  • Translational regulation
  • Vasa

ASJC Scopus subject areas

  • Embryology
  • Developmental Biology

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