Expression and characterization of a very low density lipoprotein receptor variant lacking the O-linked sugar region generated by alternative splicing

Hiroaki Iijima, Masaaki Miyazawa, Juro Sakai, Kenta Magoori, Mitsuko R. Ito, Hiroyuki Suzuki, Masato Nose, Yutaka Kawarabayasi, Tokuo T. Yamamoto

Research output: Contribution to journalArticlepeer-review

40 Citations (Scopus)

Abstract

The very low density lipoprotein receptor (VLDLR) gene contains an exon encoding a region of clustered serine and threonine residues immediately outside the membrane-spanning sequence, and this region has been proposed to be the site of clustered O-linked carbohydrate chains. Two forms of VLDLR transcripts, with and without the O-linked sugar region, are generated through alternative splicing. Reverse transcription polymerase chain reaction with RNAs from various rabbit tissues revealed that the VLDLR transcript with the O-linked sugar region (type-1 VLDLR) is the major transcript in heart and muscle, while the VLDLR transcript without the O-linked sugar region (type-2 VLDLR) predominates in non-muscle tissues, including cerebrum, cerebellum, kidney, spleen, adrenal gland, testis, ovary, and uterus. Hamster fibroblasts expressing type-2 VLDLR bound with relatively low affinity to β-migrating very low density lipoprotein compared with type-1 VLDLR-transfected cells. In contrast, the internalization, dissociation, and degradation of the ligand were not significantly impaired in either type of VLDLR-transfected cell. The receptor proteins in type-2 VLDLR-transfected cells underwent rapid degradation and accumulated in the culture medium, while those in type-1 VLDLR-transfected cells were stable and resistant to proteolytic cleavage. Analysis of the O-linked sugars of both types of transfected cells suggested that the O-linked sugar region is the major site for O-glycosylation.

Original languageEnglish
Pages (from-to)747-755
Number of pages9
JournalJournal of biochemistry
Volume124
Issue number4
DOIs
Publication statusPublished - 1998 Oct

Keywords

  • Lipoprotein
  • O-linked sugar
  • Splicing variant
  • VLDL receptor

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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