Expression analysis of LacZ gene placed in the locus of Cnot7 exhibits its activity in osteoblasts in vivo and in mineralized nodules in vitro

Kaoru Washio-Oikawa, Takahisa Nakamura, Michihiko Usui, Mitsuhiro Yoneda, Youichi Ezura, Isao Ishikawa, Kazuhisa Nakashima, Tadashi Yamamoto, Masaki Noda

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

CCR4-NOT complex 7 (Cnot7) was identified as a regulator of gene expression in yeast and evolutionally conserved in mammals. Cnot7 deficient male mice exhibit abnormality in spermatogenesis. As these mice contained construct to express LacZ, we followed the expression patterning in these animals. LacZ was expressed in osteoblasts located in the primary spongiosa in adult mice. Cellular analysis indicated that LacZ is expressed in osteoblasts but not in osteoclasts. In the mineralized nodules formed in the culture of bone marrow cells obtained from Cnot7+/- mice, LacZ expression was mainly observed in the cells forming mineralized nodules but not in un-mineralized area scattered around the periphery of the nodules. LacZ blue positive cells were gradually depositing minerals along its time course of the in vitro mineralization assay. Cnot7 expression was enhanced by the treatment with BMP. These data suggest that Cnot7 is expressed in osteoblasts and is associated with mineralization.

Original languageEnglish
Pages (from-to)538-544
Number of pages7
JournalJournal of Cellular Biochemistry
Volume99
Issue number2
DOIs
Publication statusPublished - 2006 Oct 1
Externally publishedYes

Keywords

  • Cnot7
  • LacZ
  • Mineralized nodule
  • Osteoblasts

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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