Expressed genes in the extraradical hyphae of an arbuscular mycorrhizal fungus, Glomus intraradices, in the symbiotic phase

Hiromichi Sawaki, Masanori Saito

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)

Abstract

To collect extraradical hyphae of arbuscular mycorrhizal (AM) fungi for RNA isolation, a PVDF membrane was laid on the hyphal compartment of a two-compartment culture system of transformed carrot hairy roots and Glomus intraradices. Extraradical hyphae free from host tissue were easily collected, and their RNA was rapidly extracted with a modified acid guanidinium thiocyanate-phenol-chloroform method. A 3′-RACE (rapid amplification of cDNA ends) of a known gene indicated that this protocol enabled the isolation of mRNA molecules as small as 2.3 kb. The cDNA libraries of an AM fungus from the aseptic extraradical hyphae in a symbiotic state were constructed for the first time. Three-fourth of 150 ESTs (expressed sequence tags) indicated low or no similarities to known sequences from other organisms.

Original languageEnglish
Pages (from-to)109-113
Number of pages5
JournalFEMS Microbiology Letters
Volume195
Issue number1
DOIs
Publication statusPublished - 2001 Feb 5

Keywords

  • EST
  • Glomus intraradices
  • PVDF
  • RNA isolation
  • RT-PCR
  • Symbiotic hyphae

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

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