Exploring genetic diversity and signatures of horizontal gene transfer in nodule bacteria associated with lotus japonicus in natural environments

Masaru Bamba, Seishiro Aoki, Tadashi Kajita, Hiroaki Setoguchi, Yasuyuki Watano, Shusei Sato, Takashi Tsuchimatsu

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

To investigate the genetic diversity and understand the process of horizontal gene transfer (HGT) in nodule bacteria associated with Lotus japonicus, we analyzed sequences of three housekeeping and five symbiotic genes using samples from a geographically wide range in Japan. A phylogenetic analysis of the housekeeping genes indicated that L. japonicus in natural environments was associated with diverse lineages of Mesorhizobium spp., whereas the sequences of symbiotic genes were highly similar between strains, resulting in remarkably low nucleotide diversity at both synonymous and nonsynonymous sites. Guanine-cytosine content values were lower in symbiotic genes, and relative frequencies of recombination between symbiotic genes were also lower than those between housekeeping genes. An analysis of molecular variance showed significant genetic differentiation among populations in both symbiotic and housekeeping genes. These results confirm that the Mesorhizobium genes required for symbiosis with L. japonicus behave as a genomic island (i.e., a symbiosis island) and suggest that this island has spread into diverse genomic backgrounds of Mesorhizobium via HGT events in natural environments. Furthermore, our data compilation revealed that the genetic diversity of symbiotic genes in L. japonicus-associated symbionts was among the lowest compared with reports of other species, which may be related to the recent population expansion proposed in Japanese populations of L. japonicus.

Original languageEnglish
Pages (from-to)1110-1120
Number of pages11
JournalMolecular Plant-Microbe Interactions
Volume32
Issue number9
DOIs
Publication statusPublished - 2019 Jan 1

Keywords

  • Horizontal gene transfer,
  • Rhizobium-legume symbiosis

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science

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