TY - JOUR
T1 - Exploring a DNA Sequence for the Three-Dimensional Structure Determination of a Silver(I)-Mediated C-C Base Pair in a DNA Duplex by 1H NMR Spectroscopy
AU - Dairaku, Takenori
AU - Furuita, Kyoko
AU - Sato, Hajime
AU - Kondo, Yoshinori
AU - Kojima, Chojiro
AU - Ono, Akira
AU - Tanaka, Yoshiyuki
N1 - Funding Information:
This work was performed using the NMR spectrometer under the Co-operative Research Program of the Institute for Protein Research, Osaka University. This work was supported by the Platform for Drug Discovery, Informatics, and Structural Life Science from the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan. This work was supported by Grants-in-Aid for Scientific Research (a) 24245037 to Akira Ono and Yoshiyuki Tanaka, (b) 24310163 to Yoshiyuki Tanaka and Chojiro Kojima, (c) 18550146 to Yoshiyuki Tanaka, and Challenging Exploratory Research (15K13734 to Yoshiyuki Tanaka) from MEXT, Japan. Takenori Dairaku and Kyoko Furuita are the recipients of a Research Fellowship for Young Scientists from the Japan Society for the Promotion of Science (JSPS).
Publisher Copyright:
Copyright © 2015 Taylor and Francis Group, LLC.
PY - 2015/12/2
Y1 - 2015/12/2
N2 - Recently, we discovered novel silver(I)-mediated cytosine-cytosine base pair (C-AgI-C) in DNA duplexes. To understand the properties of these base pairs, we searched for a DNA sequence that can be used in NMR structure determination. After extensive sequence optimizations, a non-symmetric 15-base-paired DNA duplex with a single C-AgI-C base pair flanked by 14 A-T base pairs was selected. In spite of its challenging length for NMR measurements (30 independent residues) with small sequence variation, we could assign most non-exchangeable protons (254 out of 270) and imino protons for structure determination.
AB - Recently, we discovered novel silver(I)-mediated cytosine-cytosine base pair (C-AgI-C) in DNA duplexes. To understand the properties of these base pairs, we searched for a DNA sequence that can be used in NMR structure determination. After extensive sequence optimizations, a non-symmetric 15-base-paired DNA duplex with a single C-AgI-C base pair flanked by 14 A-T base pairs was selected. In spite of its challenging length for NMR measurements (30 independent residues) with small sequence variation, we could assign most non-exchangeable protons (254 out of 270) and imino protons for structure determination.
KW - H NMR spectroscopy
KW - metallo-DNA
KW - metallo-base pair
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U2 - 10.1080/15257770.2015.1088160
DO - 10.1080/15257770.2015.1088160
M3 - Article
C2 - 26576739
AN - SCOPUS:84949214311
VL - 34
SP - 877
EP - 900
JO - Nucleosides, Nucleotides and Nucleic Acids
JF - Nucleosides, Nucleotides and Nucleic Acids
SN - 1525-7770
IS - 12
ER -