Abstract
Recently, we discovered novel silver(I)-mediated cytosine-cytosine base pair (C-AgI-C) in DNA duplexes. To understand the properties of these base pairs, we searched for a DNA sequence that can be used in NMR structure determination. After extensive sequence optimizations, a non-symmetric 15-base-paired DNA duplex with a single C-AgI-C base pair flanked by 14 A-T base pairs was selected. In spite of its challenging length for NMR measurements (30 independent residues) with small sequence variation, we could assign most non-exchangeable protons (254 out of 270) and imino protons for structure determination.
| Original language | English |
|---|---|
| Pages (from-to) | 877-900 |
| Number of pages | 24 |
| Journal | Nucleosides, Nucleotides and Nucleic Acids |
| Volume | 34 |
| Issue number | 12 |
| DOIs | |
| Publication status | Published - 2015 Dec 2 |
Keywords
- H NMR spectroscopy
- metallo-DNA
- metallo-base pair
ASJC Scopus subject areas
- Biochemistry
- Molecular Medicine
- Genetics
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Cite this
Dairaku, T., Furuita, K., Sato, H., Kondo, Y., Kojima, C., Ono, A., & Tanaka, Y. (2015). Exploring a DNA Sequence for the Three-Dimensional Structure Determination of a Silver(I)-Mediated C-C Base Pair in a DNA Duplex by 1H NMR Spectroscopy. Nucleosides, Nucleotides and Nucleic Acids, 34(12), 877-900. https://doi.org/10.1080/15257770.2015.1088160