Exploration of bacterial N2-fixation systems in association with soil-grown sugarcane, sweet potato, and paddy rice: a review and synthesis

Tadakatsu Yoneyama, Junko Terakado-Tonooka, Kiwamu Minamisawa

    Research output: Contribution to journalReview article

    10 Citations (Scopus)

    Abstract

    N2 fixation systems in the nonleguminous crops and bacteria associations have been intensively studied over the last 50 years. Their structure and regulation have been investigated to explore the enhancement of N acquisition in these ecosystems leading to crop-growth with minimum chemical fertilizers. Several lines of important evidence have been accumulated indicating that the magnitudes of associative (nonsymbiotic) N2 fixation in sugarcane (Saccharum spp.), sweet potato (Ipomoea batatas L.), and paddy rice (Oryza sativa L.) are agronomically significant. In these three crops, unique bacterial N2-fixation systems may function in addition to the low-level activity (due to the competition in carbon/energy use) of the commonly occurring rhizosphere-associated system by free-living bacteria such as Beijerinckia, Azotobacter, and Klebsiella. Active expressions of the dinitrogenase reductase-encoded gene (nifH) phylogenetically similar to those of Bradyrhizobium spp. and Azorhizobium sp. were abundantly found in the N2-fixing sugarcane stems, sweet potato stems, and storage tubers. These rhizobia micro-aerobically fix N2 in the carbon compounds-rich apoplasts. Gluconacetobacter diazotrophicus and Herbaspirillum spp. were previously isolated from inside the sugarcane stems, as the candidates of endophytic N2 fixers. However, the current molecular and physiological investigations suggest that their major role is production of phytohormonal substances. In paddy rice fields, methane is produced from organic compounds in anoxia and oxidized by contacting with oxygen gas. An active N2-fixation by methane-oxidizing methanotrophs such as Methylosinus sp. takes place in the root tissues (aerenchyma) and also in the surface soil. This methanotrophic N2-fixation supports the sustainability of soil fertility although the N2-fixation and soil fertility are affected by chemical fertilizers. Finally, we discuss the ecological implications of the newly identified rhizobia and methanotroph systems in the N nutrition in nonlegumes and N reservation in field environments.

    Original languageEnglish
    Pages (from-to)578-590
    Number of pages13
    JournalSoil Science and Plant Nutrition
    Volume63
    Issue number6
    DOIs
    Publication statusPublished - 2017 Nov 2

    Keywords

    • Methanotroph
    • rhizobia
    • rice (Oryza sativa L.)
    • sugarcane (Saccharum sp.)
    • sweet potato (Ipomoea batatas L.)

    ASJC Scopus subject areas

    • Soil Science
    • Plant Science

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