The luminol (5-amlno-2,3-cHhyclro-1,4-phthalazlnedlone)-hydrogen peroxide-3-amlnophthalate chemiluminescence (CL) system was characterized In reversed micelles of hexa-decyltrlmethylammonlum chloride (CTAC), formed In 6:5 (v/v) chloroform-cyclohexane. The relative effectiveness of this medium for analytical CL measurements was assessed. Whereas no light emission was observed from the lumlnol-hydrogen peroxide CL reaction in bulk aqueous solution at mild pH (7.8-9.1) In the absence of any added catalyst or cooxldant, Intense CL was observed In the CTAC reversed micellar system. The effect of experimental variables (l.e., surfactant concentration, cosoiublllzed water, luminol concentration) upon the CL Intenaliy was evaluated. In the CTAC reversed micellar medium, the luminol CL assay can be applied to the analysis of hydrogen peroxide In the 6.4 × 10-7 to 6.4 × 10-5 M concentration range. The precision of the reversed micellar modified procedure Is quite satisfactory, ranging from 0.9 to 12.3% in terms of the relative standard deviation over the range of peroxide concentrations examined. The feasibility of using the reversed micellar effect to determine enzymes or substrates In coupled reaction systems that produce hydrogen peroxide Is discussed.
ASJC Scopus subject areas
- Analytical Chemistry