In this paper, we report the experimental study on novel DNA separation using electrode-embedded microchannel. A microchannel with an interdigitated electrode on the wall was developed for investigation. The experiment was carried out with flowing solution including λDNA by pressure driven flow. DC voltage of ∼2 V was applied to the interdigitated electrode. Under the ideal condition, λDNA is trapped on electrode and released from it stochastically. From the observation, it was found that the velocity of λDNA was decreased by the electric field when it approached to a positive electrode. Furthermore, we theoretically estimated the trajectory of flowing λDNA in the microchannel. Comparing the experimental and theoretical results, it was found that the electric field by the interdigitated electrodes was shielded by electrolyte in the solution.