Experimental phase determination with selenomethionine or mercury-derivatization in serial femtosecond crystallography

Keitaro Yamashita, Naoyuki Kuwabara, Takanori Nakane, Tomohiro Murai, Eiichi Mizohata, Michihiro Sugahara, Dongqing Pan, Tetsuya Masuda, Mamoru Suzuki, Tomomi Sato, Atsushi Kodan, Tomohiro Yamaguchi, Eriko Nango, Tomoyuki Tanaka, Kensuke Tono, Yasumasa Joti, Takashi Kameshima, Takaki Hatsui, Makina Yabashi, Hiroshi ManyaTamao Endo, Ryuichi Kato, Toshiya Senda, Hiroaki Kato, So Iwata, Hideo Ago, Masaki Yamamoto, Fumiaki Yumoto, Toru Nakatsu

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Serial femtosecond crystallography (SFX) using X-ray free-electron lasers (XFELs) holds enormous potential for the structure determination of proteins for which it is difficult to produce large and high-quality crystals. SFX has been applied to various systems, but rarely to proteins that have previously unknown structures. Consequently, the majority of previously obtained SFX structures have been solved by the molecular replacement method. To facilitate protein structure determination by SFX, it is essential to establish phasing methods that work efficiently for SFX. Here, selenomethionine derivatization and mercury soaking have been investigated for SFX experiments using the high-energy XFEL at the SPring-8 Angstrom Compact Free-Electron Laser (SACLA), Hyogo, Japan. Three successful cases are reported of single-wavelength anomalous diffraction (SAD) phasing using X-rays of less than 1Å wavelength with reasonable numbers of diffraction patterns (13000, 60000 and 11000). It is demonstrated that the combination of high-energy X-rays from an XFEL and commonly used heavy-atom incorporation techniques will enable routine de novo structural determination of biomacromolecules.

Original languageEnglish
Pages (from-to)639-647
Number of pages9
JournalIUCrJ
Volume4
DOIs
Publication statusPublished - 2017
Externally publishedYes

Keywords

  • SAD phasing
  • XFELs
  • mercury soaking
  • selenomethionine derivatization
  • serial femtosecond crystallography

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry
  • Materials Science(all)
  • Condensed Matter Physics

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  • Cite this

    Yamashita, K., Kuwabara, N., Nakane, T., Murai, T., Mizohata, E., Sugahara, M., Pan, D., Masuda, T., Suzuki, M., Sato, T., Kodan, A., Yamaguchi, T., Nango, E., Tanaka, T., Tono, K., Joti, Y., Kameshima, T., Hatsui, T., Yabashi, M., ... Nakatsu, T. (2017). Experimental phase determination with selenomethionine or mercury-derivatization in serial femtosecond crystallography. IUCrJ, 4, 639-647. https://doi.org/10.1107/S2052252517008557