Experimental model of tooth movement by orthodontic force in mice and its application to tumor necrosis factor receptor-deficient mice

Masako Yoshimatsu, Yasuaki Shibata, Hideki Kitaura, Xin Chang, Takeshi Moriishi, Fumio Hashimoto, Noriaki Yoshida, Akira Yamaguchi

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

Orthodontic tooth movement is achieved by mechanical loading; however, the biological mechanism involved in this process is not clearly understood owing to the lack of a suitable experimental model. In the present study, we established an orthodontic tooth movement model in mice using a Ni-Ti closed coil spring that was inserted between the upper incisors and the upper first molar. Histological examination demonstrated that the orthodontic force moved the first upper molar mesially without necrosis of the periodontium during tooth movement. The number of TRAP-positive osteoclasts on the pressure side significantly increased in a time-dependent manner. Quantitative real time-based reverse transcription-polymerase chain reaction analysis demonstrated increased levels of mRNA for cathepsin K. Immunohistochemical staining revealed the expression of tumor necrosis factor-α (TNFα) in periodontium on the pressure side of the first molar during orthodontic tooth movement. When this tooth movement system was applied to TNF type 1 receptor-deficient mice and TNF type 2 receptor-deficient mice, tooth movement observed in TNF type 2 receptor-deficient mice was smaller than that in the wild-type mice and TNF type 1 receptor-deficient mice. The number of TRAP-positive osteoclasts on the pressure side was significantly small in TNF type 2 receptor-deficient mice compared with that in TNF type 1 receptor-deficient mice on day 6 after application of the appliance. The present study indicates that TNFα signaling plays some important roles in orthodontic tooth movement.

Original languageEnglish
Pages (from-to)20-27
Number of pages8
JournalJournal of Bone and Mineral Metabolism
Volume24
Issue number1
DOIs
Publication statusPublished - 2006 Jan 1
Externally publishedYes

Keywords

  • Bone
  • Bone resorption
  • Mechanical loading
  • Mouse
  • Orthodontic tooth movement
  • TNFα

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Orthopedics and Sports Medicine
  • Endocrinology

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