Expansion of triplex recognition codes by the use of novel bicyclic nucleoside derivatives (WNA)

Yosuke Taniguchi; Ayako Nakamura; Yusuke Senko; Keiichi Kodama; Fumi Nagatsugi; Shigeki Sasaki

doi:10.1081/NCN-200060309

Expansion of triplex recognition codes by the use of novel bicyclic nucleoside derivatives (WNA)

Yosuke Taniguchi, Ayako Nakamura, Yusuke Senko, Keiichi Kodama, Fumi Nagatsugi, Shigeki Sasaki

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

Recently, we have developed new base analogs (WNA) and demonstrated that WNA-&#6T538;T with thymine and WNA-C with cytosine stabilize non-natural antiparallel triplexes with a TA or a CG interrupting site, respectively. However, limitations in recognizable sequences with the WNA-containing TFO were also found. The objective of this study is to search better WNA analogs for expansion of triplex recognition codes to general duplex sequences. In this study, we designed new WNA analogs by systematic modification of the aromatic part and the recognition part. The new WNA analogs with the benzene ring substituted with bromide or cyanide have determined for selective stabilization of triplexes at a TA interrupting site, and general formation of triplexes having a TA interrupting site has been achieved.

Original language	English
Pages (from-to)	823-827
Number of pages	5
Journal	Nucleosides, Nucleotides and Nucleic Acids
Volume	24
Issue number	5-7
DOIs	https://doi.org/10.1081/NCN-200060309
Publication status	Published - 2005 Oct 20
Externally published	Yes

Keywords

Antigene
Interrupting Site
Molecular Recognition
Non-Natural Nucleoside
Triplex DNA

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Genetics

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abstract = "Recently, we have developed new base analogs (WNA) and demonstrated that WNA-&#6T538;T with thymine and WNA-C with cytosine stabilize non-natural antiparallel triplexes with a TA or a CG interrupting site, respectively. However, limitations in recognizable sequences with the WNA-containing TFO were also found. The objective of this study is to search better WNA analogs for expansion of triplex recognition codes to general duplex sequences. In this study, we designed new WNA analogs by systematic modification of the aromatic part and the recognition part. The new WNA analogs with the benzene ring substituted with bromide or cyanide have determined for selective stabilization of triplexes at a TA interrupting site, and general formation of triplexes having a TA interrupting site has been achieved.",

keywords = "Antigene, Interrupting Site, Molecular Recognition, Non-Natural Nucleoside, Triplex DNA",

author = "Yosuke Taniguchi and Ayako Nakamura and Yusuke Senko and Keiichi Kodama and Fumi Nagatsugi and Shigeki Sasaki",

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T1 - Expansion of triplex recognition codes by the use of novel bicyclic nucleoside derivatives (WNA)

AU - Taniguchi, Yosuke

AU - Nakamura, Ayako

AU - Senko, Yusuke

AU - Kodama, Keiichi

AU - Nagatsugi, Fumi

AU - Sasaki, Shigeki

PY - 2005/10/20

Y1 - 2005/10/20

N2 - Recently, we have developed new base analogs (WNA) and demonstrated that WNA-&#6T538;T with thymine and WNA-C with cytosine stabilize non-natural antiparallel triplexes with a TA or a CG interrupting site, respectively. However, limitations in recognizable sequences with the WNA-containing TFO were also found. The objective of this study is to search better WNA analogs for expansion of triplex recognition codes to general duplex sequences. In this study, we designed new WNA analogs by systematic modification of the aromatic part and the recognition part. The new WNA analogs with the benzene ring substituted with bromide or cyanide have determined for selective stabilization of triplexes at a TA interrupting site, and general formation of triplexes having a TA interrupting site has been achieved.

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KW - Interrupting Site

KW - Molecular Recognition

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KW - Triplex DNA

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