Exogenous addition of histidine reduces copper availability in the yeast Saccharomyces cerevisiae

Daisuke Watanabe, Rie Kikushima, Miho Aitoku, Akira Nishimura, Iwao Ohtsu, Ryo Nasuno, Hiroshi Takagi

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)


The basic amino acid histidine inhibited yeast cell growth more severely than lysine and arginine. Overexpression of CTR1, which encodes a high-affinity copper transporter on the plasma membrane, or addition of copper to the medium alleviated this cytotoxicity. However, the intracellular level of copper ions was not decreased in the presence of excess histidine. These results indicate that histidine cytotoxicity is associated with low copper availability inside cells, not with impaired copper uptake. Furthermore, histidine did not affect cell growth under limited respiration conditions, suggesting that histidine cytotoxicity is involved in deficiency of mitochondrial copper.

Original languageEnglish
Pages (from-to)241-246
Number of pages6
JournalMicrobial Cell
Issue number7
Publication statusPublished - 2014 Jul
Externally publishedYes


  • Basic amino acids
  • Copper transporter Ctr1
  • Histidine cytotoxicity
  • Mitochondrial respiration
  • Yeast Saccharomyces cerevisiae

ASJC Scopus subject areas

  • Immunology and Microbiology (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology (miscellaneous)


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