Existence of GPR40 functioning in a human breast cancer cell line, MCF-7

T. Yonezawa, K. Katoh, Y. Obara

    Research output: Contribution to journalArticlepeer-review

    77 Citations (Scopus)

    Abstract

    GPR40, which has recently been identified as a G-protein-coupled cell-surface receptor for long-chain fatty acids, was assessed in a human breast cancer cell line (MCF-7). We detected GPR40 mRNA by RT-PCR and found that oleate and linoleate, but not palmitate or stearate, caused an increase in cellular Ca2+ concentrations, which was partially blocked by the pertussis toxin (PTX) treatment. We examined the expression of GPR40 mRNA by quantitative RT-PCR in the relation to cell number. It was significantly increased at the beginning and at the end of cell proliferation. These results indicate the possibility that GPR40 for long-chain fatty acids may be involved in cellular function such as cell proliferation, providing a new perspective for the action of long-chain fatty acids on mammary epithelial cells.

    Original languageEnglish
    Pages (from-to)805-809
    Number of pages5
    JournalBiochemical and biophysical research communications
    Volume314
    Issue number3
    DOIs
    Publication statusPublished - 2004 Feb 13

    Keywords

    • Breast cancer cell
    • GPR40
    • Intracellular calcium
    • Long-chain fatty acids

    ASJC Scopus subject areas

    • Biophysics
    • Biochemistry
    • Molecular Biology
    • Cell Biology

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