Abstract
GPR40, which has recently been identified as a G-protein-coupled cell-surface receptor for long-chain fatty acids, was assessed in a human breast cancer cell line (MCF-7). We detected GPR40 mRNA by RT-PCR and found that oleate and linoleate, but not palmitate or stearate, caused an increase in cellular Ca2+ concentrations, which was partially blocked by the pertussis toxin (PTX) treatment. We examined the expression of GPR40 mRNA by quantitative RT-PCR in the relation to cell number. It was significantly increased at the beginning and at the end of cell proliferation. These results indicate the possibility that GPR40 for long-chain fatty acids may be involved in cellular function such as cell proliferation, providing a new perspective for the action of long-chain fatty acids on mammary epithelial cells.
Original language | English |
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Pages (from-to) | 805-809 |
Number of pages | 5 |
Journal | Biochemical and biophysical research communications |
Volume | 314 |
Issue number | 3 |
DOIs | |
Publication status | Published - 2004 Feb 13 |
Keywords
- Breast cancer cell
- GPR40
- Intracellular calcium
- Long-chain fatty acids
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology