Existence and expression of photoreactivation repair genes in various yeast species

A. Yasui, A. P.M. Eker, M. Koken

    Research output: Contribution to journalArticlepeer-review

    9 Citations (Scopus)

    Abstract

    Photoreactivation repair (Phr) activities in cell extracts of 13 different yeast specie were measured by the Haemophilus influenzae transformation assay. Five species including Schizosaccharomyces pombe showed no or low enzymatic activity. In contrast to the other species, chromosomal DNAs of these 5 species did not show detectable hybridization using a DNA fragment of the photolyase PHR1 gene of Saccharomyces cervisiae as a probe even at a low stringency condition. When the PHR1 gene was attached to the 5′-flanking sequence of the iso-1-cytochrome c (CYC-1) gene of S. cerevisiae and introduced into S. pombe cells, the transformants acquired a high Phr activity, indicating that the PHR1 gene alone can provide a Phr-negative species with this repair activity and the light-absorbing cofactor(s) must be present in S. pombe. Our results also demonstrated that the 5′-flanking sequence of the S. cerevisiae CYC-1 gene works in S. pombe as a regulatory element.

    Original languageEnglish
    Pages (from-to)3-10
    Number of pages8
    JournalMutation Research-DNA Repair
    Volume217
    Issue number1
    DOIs
    Publication statusPublished - 1989 Jan

    Keywords

    • Hybridization of DNA repair gene
    • Photolyase
    • Schizosaccharomyces pombe
    • Yeast species

    ASJC Scopus subject areas

    • Molecular Biology
    • Toxicology
    • Genetics

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