Examination of the association states of dehydroergosterol towards understanding the association structures of sterols in a membrane

Kunisato Kuroi, Masatomo Imaizumi, Takashi Miura, Takakazu Nakabayashi

Research output: Contribution to journalArticle

Abstract

Cholesterol plays a number of roles in cell membranes, and dehydroergosterol (DHE) is a fluorescent derivative of cholesterol, which is used to investigate the association structure of cholesterol. Although the fluorescent property of DHE depends on its association state, it is insufficient to distinguish the association state of DHE only by its fluorescence. Circular dichroism (CD) spectroscopy is an effective way to investigate the molecular geometry of DHE. In the present study, therefore we investigated the association structure of DHE by CD and fluorescence spectroscopy in solution and in a lipid membrane. DHE was shown to exist as three states (monomeric, microcrystalline, and micelle states) in methanol-water mixtures depending on solvent hydrophobicity. The CD spectrum of DHE in a liposome was similar to that of the micelle state, indicating that the association state of DHE in a liposome exhibits a parallel arrangement similar to that in the micelle state. This result is difficult to be obtained only from the measurement of the fluorescence spectra. The combination of CD and fluorescence spectroscopic techniques is necessary to investigate the association of DHE.

Original languageEnglish
Pages (from-to)228-233
Number of pages6
JournalBiochemical and biophysical research communications
Volume515
Issue number1
DOIs
Publication statusPublished - 2019 Jul 12

Keywords

  • Association structure
  • Cholesterol
  • Circular dichroism
  • Dehydroergosterol
  • Fluorescence
  • Membrane

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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