Ex vivo genome-wide RNAi screening of the Drosophila Toll signaling pathway elicited by a larva-derived tissue extract

Hirotaka Kanoh, Takayuki Kuraishi, Li Li Tong, Ryo Watanabe, Shinji Nagata, Shoichiro Kurata

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Damage-associated molecular patterns (DAMPs), so-called "danger signals," play important roles in host defense and pathophysiology in mammals and insects. In Drosophila, the Toll pathway confers damage responses during bacterial infection and improper cell-fate control. However, the intrinsic ligands and signaling mechanisms that potentiate innate immune responses remain unknown. Here, we demonstrate that a Drosophila larva-derived tissue extract strongly elicits Toll pathway activation via the Toll receptor. Using this extract, we performed ex vivo genome-wide RNAi screening in Drosophila cultured cells, and identified several signaling factors that are required for host defense and antimicrobial-peptide expression in Drosophila adults. These results suggest that our larva-derived tissue extract contains active ingredients that mediate Toll pathway activation, and the screening data will shed light on the mechanisms of damage-related Toll pathway signaling in Drosophila.

Original languageEnglish
Pages (from-to)400-406
Number of pages7
JournalBiochemical and biophysical research communications
Volume467
Issue number2
DOIs
Publication statusPublished - 2015 Nov 13

Keywords

  • Danger signals
  • Drosophila
  • Innate immunity
  • Sterile inflammation
  • Toll pathway

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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