Abstract
The apolipoprotein E receptor 2 (apoER2) gene consists of a mosaic of exons, which may have been assembled by 'exon shuffling.' Analysis of apoER2 transcripts in several species reveals a lost repeat in the ligand-binding domain of primate apoER2. A pseudo-exon found in the primate apoER2 genes corresponds to the lost repeat but contains a crucial deletion that leads to a translational frameshift. The pseudo-exon sequence in primary transcripts of the human apoER2 gene is shown to be abolished by exon skipping due to two nucleotide substitutions at the 5'-splice donor adjacent to the pseudo-exon. These data suggest the occurrence of exon loss in the evolution of the primate apoER2 gene.
Original language | English |
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Pages (from-to) | 451-456 |
Number of pages | 6 |
Journal | Journal of biochemistry |
Volume | 124 |
Issue number | 2 |
DOIs | |
Publication status | Published - 1998 Aug |
Externally published | Yes |
Keywords
- Exon shuffling
- Exon skipping
- Pseudo exon
- Repetitive sequence
- apoE receptor
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology