Evolution and regulation of the lotus japonicus LysM receptor gene family

Gitte Vestergaard Lohmann, Yoshikazu Shimoda, Mette Wibroe Nielsen, Frank Grønlund Jorgensen, Christina Grossmann, Niels Sandal, Kirsten Sørensen, Soren Thirup, Lene Heegaard Madsen, Satoshi Tabata, Shusei Sato, Jens Stougaard, Simona Radutoiu

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72 Citations (Scopus)

Abstract

LysM receptor kinases were identified as receptors of acy-lated chitin (Nod factors) or chitin produced by plant-interacting microbes. Here, we present the identification and characterization of the LysM receptor kinase gene (Lys) family (17 members) in Lotus japonicus. Comprehensive phylogenetic analysis revealed a correlation between Lys gene structure and phylogeny. Further mapping coupled with sequence-based anchoring on the genome showed that the family has probably expanded by a combination of tan-dem and segmental duplication events. Using a sliding-win-dow approach, we identified distinct regions in the LysM and kinase domains of recently diverged Lys genes where positive selection may have shaped ligand interaction. Inter-estingly, in the case of NFR5 and its closest paralog, LYS11, one of these regions coincides with the predicted Nod-factor binding groove and the suggested specificity determining area of the second LysM domain. One hypothesis for the evolutionary diversification of this receptor family in leg-umes is their unique capacity to decipher various struc-tures of chitin-derived molecules produced by an extended spectrum of interacting organisms: symbiotic, associative, endophytic, and parasitic. In a detailed expression analysis, we found several Lotus Lys genes regulated not only during the symbiotic association with Mesorhizobium loti but also in response to chitin treatment.

Original languageEnglish
Pages (from-to)510-521
Number of pages12
JournalMolecular Plant-Microbe Interactions
Volume23
Issue number4
DOIs
Publication statusPublished - 2010 Apr 1
Externally publishedYes

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science

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    Lohmann, G. V., Shimoda, Y., Nielsen, M. W., Jorgensen, F. G., Grossmann, C., Sandal, N., Sørensen, K., Thirup, S., Madsen, L. H., Tabata, S., Sato, S., Stougaard, J., & Radutoiu, S. (2010). Evolution and regulation of the lotus japonicus LysM receptor gene family. Molecular Plant-Microbe Interactions, 23(4), 510-521. https://doi.org/10.1094/MPMI-23-4-0510