Evidence of IgG‐mediated enhancement of the antibody response in vivo without complement activation via the classical pathway

Erik J. Wiersma, Masato Nose, Brigitta Heyman

Research output: Contribution to journalArticlepeer-review

34 Citations (Scopus)

Abstract

The complement (C) dependency of IgG‐mediated enhancement of the antibody response was investigated by immunizing mice with trinitrophenyl‐coupled keyhole limpet hemocyanin (TNP‐KLH) and either a C‐activating TNP‐specific monoclonal IgG2a antibody (Hy‐1.2) or a mutant, non‐C‐activating variant of Hy‐1.2 (M12). Hy‐1.2 as well as M12 efficiently enhanced the anti‐KLH response, although Hy‐1.2 was more active. In addition, also a naturally non‐C‐activating TNP‐specific IgG1 antibody enhanced the response to TNP‐coupled bovine serum albumin. Moreover, C‐activating IgG could enhance the antibody response in mice depleted of C3 by treatment with cobra venom factor. These findings suggest that the classical pathway of C activation is not required for IgG‐mediated enhancement.

Original languageEnglish
Pages (from-to)2585-2589
Number of pages5
JournalEuropean Journal of Immunology
Volume20
Issue number12
DOIs
Publication statusPublished - 1990 Dec

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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