Using a cellular approach, the present study examined whether fasudil and active metabolite hydroxyfasudil, Rho-kinase inhibitors, exert a direct protective effect on endothelin-induced cardiac myocyte hypertrophy in vitro. Treatment with endothelin (10. nM) caused significant hypertrophy of cultured neonatal rat cardiomyocytes by a 21.2% increase in cell surface area. Fasudil (1-10. μM) and hydroxyfasudil (0.3-10. μM) significantly prevented endothelin-induced cardiomyocyte hypertrophy. The present results suggest that inhibition of cardiac hypertrophy by fasudil is, at least in part, due to direct protection of cardiomyocytes from hypertrophy.
|Number of pages||3|
|Journal||Biochemical and biophysical research communications|
|Publication status||Published - 2012 Jul 27|
- Cardiomyocyte hypertrophy
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology