Evidence for motoneuron lineage-specific regulation of Olig2 in the vertebrate neural tube

Tao Sun, Brian P. Hafler, Sovann Kaing, Masaaki Kitada, Keith L. Ligon, Hans R. Widlund, Dong in Yuk, Charles D. Stiles, David H. Rowitch

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

Within the motoneuron precursor (pMN) domain of the developing spinal cord, the bHLH transcription factor, Olig2, plays critical roles in pattern formation and the generation of motor neuron and oligodendrocyte precursors. How are the multiple functions of Olig2 regulated? We have isolated a large BAC clone encompassing the human OLIG2 locus that rescues motor neuron and oligodendrocyte development but not normal pattern formation in Olig2-/- embryos. Within the BAC clone, we identified a conserved 3.6 kb enhancer sub-region that directs reporter expression specifically in the motor neuron lineage but not oligodendrocyte lineage in vivo. Our findings indicate complex regulation of Olig2 by stage- and lineage-specific regulatory elements. They further suggest that transcriptional regulation of Olig2 is involved in segregation of pMN neuroblasts.

Original languageEnglish
Pages (from-to)152-164
Number of pages13
JournalDevelopmental Biology
Volume292
Issue number1
DOIs
Publication statusPublished - 2006 Apr 1

Keywords

  • Bacterial Artificial Chromosome (BAC)
  • Enhancer
  • Lineage mapping
  • Motor neuron
  • Neural tube
  • Olig2
  • Oligodendrocyte
  • Transcription factor
  • cis-regulation

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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