Evidence for a role of C-terminus in Ca2+ inactivation of skeletal muscle Ca2+ release channel (ryanodine receptor)

Junichi Nakai, Ling Gao, Le Xu, Chunlin Xin, Daniel A. Pasek, Gerhard Meissner

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Six chimeras of the skeletal muscle (RyR1) and cardiac muscle (RyR2) Ca2+ release channels (ryanodine receptors) previously used to identify RyR1 dihydropyridine receptor interactions [Nakai et al. (1998) J. Biol. Chem. 273, 13403] were expressed in HEK293 cells to assess their Ca2+ dependence in [3H]ryanodine binding and single channel measurements. The results indicate that the C-terminal one-fourth has a major role in Ca2+ activation and inactivation of RyR1. Further, our results show that replacement of RyR1 regions with corresponding RyR2 regions can result in loss and/or reduction of [3H]ryanodine binding affinity while maintaining channel activity.

Original languageEnglish
Pages (from-to)154-158
Number of pages5
JournalFEBS Letters
Volume459
Issue number2
DOIs
Publication statusPublished - 1999 Oct 8
Externally publishedYes

Keywords

  • Ca release channel
  • Cardiac muscle
  • HEK293 cell
  • Ryanodine receptor
  • Skeletal muscle

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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