Evaluation of transfection efficiency in skeletal muscle using nano/microbubbles and ultrasound

Tetsuya Kodama, Atsuko Aoi, Yukiko Watanabe, Sachiko Horie, Mizuho Kodama, Li Li, Rui Chen, Noriyoshi Teramoto, Hidehiro Morikawa, Shiro Mori, Manabu Fukumoto

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)

Abstract

Recent studies have revealed that ultrasound contrast agents with low-intensity ultrasound, namely, sonoporation, can noninvasively deliver therapeutic molecules into target sites. However, the efficiency of molecular delivery is relatively low and the methodology requires optimization. Here, we investigated three types of nano/microbubbles (NMBs)-human albumin shell bubbles, lipid bubbles and acoustic liposomes-to evaluate the efficiency of gene expression in skeletal muscle as a function of their physicochemical properties and the number of bubbles in solution. We found that acoustic liposomes showed the highest transfection and gene expression efficiency among the three types of NMBs under ultrasound-optimized conditions. Liposome transfection efficiency increased with bubble volume concentration; however, neither bubble volume concentration nor their physicochemical properties were related to the tissue damage detected in the skeletal muscle, which was primarily caused by needle injection. (E-mail: kodama@bme.tohoku.ac.jp).

Original languageEnglish
Pages (from-to)1196-1205
Number of pages10
JournalUltrasound in Medicine and Biology
Volume36
Issue number7
DOIs
Publication statusPublished - 2010 Jul

Keywords

  • DDS
  • Nanomedicine
  • Sonoporation

ASJC Scopus subject areas

  • Biophysics
  • Radiological and Ultrasound Technology
  • Acoustics and Ultrasonics

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