Evaluation of the stability of hydrogen production and microbial diversity by anaerobic sludge with chloroform treatment

Yan Yan Ning; Da Wei Jin; Guo Ping Sheng; Hideki Harada; Xian Yang Shi

doi:10.1016/j.renene.2011.07.038

Evaluation of the stability of hydrogen production and microbial diversity by anaerobic sludge with chloroform treatment

Yan Yan Ning, Da Wei Jin, Guo Ping Sheng, Hideki Harada, Xian Yang Shi

New Industry Creation Hatchery Center (NICHe)

Research output: Contribution to journal › Article

17 Citations (Scopus)

Abstract

The stability of biological H₂ production and microbial diversity by anaerobic sludge treated chloroform with different concentrations were investigated in this work. Taking into account the cumulative H₂ production, H₂ percentage, maximum H₂ production rate, H₂ yield and substrate utilization efficiency together, 0.050% of CHCl₃ concentration was found to be appropriate for enhancing anaerobic H₂ production. The repeated batch tests revealed a good stability of H₂ production with the inocula treated by chloroform at different concentrations. Denaturing gradient gel electrophoresis (DGGE) analysis demonstrates that CHCl₃ showed desirable selective inhibition on methanogens, rather than H₂-producing bacteria. The favorable microbial communities for fermentative H₂ production by anaerobic sludge were uncultured bacterium clones nbt176c01, Janthinobacterium sp.7, Megasphaera paucivorans strain VTT E-03234, Clostridium cellulosi strain D3 and Clostridium sp. HPB-4.

Original language	English
Pages (from-to)	253-257
Number of pages	5
Journal	Renewable Energy
Volume	38
Issue number	1
DOIs	https://doi.org/10.1016/j.renene.2011.07.038
Publication status	Published - 2012 Feb 1

Keywords

Anaerobic H production
CHCl treatment
PCR-DGGE

ASJC Scopus subject areas

Renewable Energy, Sustainability and the Environment

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Ning, Y. Y., Jin, D. W., Sheng, G. P., Harada, H., & Shi, X. Y. (2012). Evaluation of the stability of hydrogen production and microbial diversity by anaerobic sludge with chloroform treatment. Renewable Energy, 38(1), 253-257. https://doi.org/10.1016/j.renene.2011.07.038

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abstract = "The stability of biological H2 production and microbial diversity by anaerobic sludge treated chloroform with different concentrations were investigated in this work. Taking into account the cumulative H2 production, H2 percentage, maximum H2 production rate, H2 yield and substrate utilization efficiency together, 0.050% of CHCl3 concentration was found to be appropriate for enhancing anaerobic H2 production. The repeated batch tests revealed a good stability of H2 production with the inocula treated by chloroform at different concentrations. Denaturing gradient gel electrophoresis (DGGE) analysis demonstrates that CHCl3 showed desirable selective inhibition on methanogens, rather than H2-producing bacteria. The favorable microbial communities for fermentative H2 production by anaerobic sludge were uncultured bacterium clones nbt176c01, Janthinobacterium sp.7, Megasphaera paucivorans strain VTT E-03234, Clostridium cellulosi strain D3 and Clostridium sp. HPB-4.",

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