Evaluation of the stability of hydrogen production and microbial diversity by anaerobic sludge with chloroform treatment

Yan Yan Ning; Da Wei Jin; Guo Ping Sheng; Hideki Harada; Xian Yang Shi

doi:10.1016/j.renene.2011.07.038

Evaluation of the stability of hydrogen production and microbial diversity by anaerobic sludge with chloroform treatment

Yan Yan Ning, Da Wei Jin, Guo Ping Sheng, Hideki Harada, Xian Yang Shi

New Industry Creation Hatchery Center (NICHe)

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

The stability of biological H₂ production and microbial diversity by anaerobic sludge treated chloroform with different concentrations were investigated in this work. Taking into account the cumulative H₂ production, H₂ percentage, maximum H₂ production rate, H₂ yield and substrate utilization efficiency together, 0.050% of CHCl₃ concentration was found to be appropriate for enhancing anaerobic H₂ production. The repeated batch tests revealed a good stability of H₂ production with the inocula treated by chloroform at different concentrations. Denaturing gradient gel electrophoresis (DGGE) analysis demonstrates that CHCl₃ showed desirable selective inhibition on methanogens, rather than H₂-producing bacteria. The favorable microbial communities for fermentative H₂ production by anaerobic sludge were uncultured bacterium clones nbt176c01, Janthinobacterium sp.7, Megasphaera paucivorans strain VTT E-03234, Clostridium cellulosi strain D3 and Clostridium sp. HPB-4.

Original language	English
Pages (from-to)	253-257
Number of pages	5
Journal	Renewable Energy
Volume	38
Issue number	1
DOIs	https://doi.org/10.1016/j.renene.2011.07.038
Publication status	Published - 2012 Feb

Keywords

Anaerobic H production
CHCl treatment
PCR-DGGE

ASJC Scopus subject areas

Renewable Energy, Sustainability and the Environment

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.renene.2011.07.038

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title = "Evaluation of the stability of hydrogen production and microbial diversity by anaerobic sludge with chloroform treatment",

abstract = "The stability of biological H2 production and microbial diversity by anaerobic sludge treated chloroform with different concentrations were investigated in this work. Taking into account the cumulative H2 production, H2 percentage, maximum H2 production rate, H2 yield and substrate utilization efficiency together, 0.050% of CHCl3 concentration was found to be appropriate for enhancing anaerobic H2 production. The repeated batch tests revealed a good stability of H2 production with the inocula treated by chloroform at different concentrations. Denaturing gradient gel electrophoresis (DGGE) analysis demonstrates that CHCl3 showed desirable selective inhibition on methanogens, rather than H2-producing bacteria. The favorable microbial communities for fermentative H2 production by anaerobic sludge were uncultured bacterium clones nbt176c01, Janthinobacterium sp.7, Megasphaera paucivorans strain VTT E-03234, Clostridium cellulosi strain D3 and Clostridium sp. HPB-4.",

keywords = "Anaerobic H production, CHCl treatment, PCR-DGGE",

author = "Ning, {Yan Yan} and Jin, {Da Wei} and Sheng, {Guo Ping} and Hideki Harada and Shi, {Xian Yang}",

note = "Funding Information: The authors wish to thank the Natural Science Key Foundation of the Anhui Higher Education Institutions ( KJ2011A003 ), and the NSFC–JST Joint Project ( 20610002 ) for the support of this study.",

year = "2012",

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doi = "10.1016/j.renene.2011.07.038",

language = "English",

volume = "38",

pages = "253--257",

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AU - Ning, Yan Yan

AU - Jin, Da Wei

AU - Sheng, Guo Ping

AU - Harada, Hideki

AU - Shi, Xian Yang

N1 - Funding Information: The authors wish to thank the Natural Science Key Foundation of the Anhui Higher Education Institutions ( KJ2011A003 ), and the NSFC–JST Joint Project ( 20610002 ) for the support of this study.

PY - 2012/2

Y1 - 2012/2

N2 - The stability of biological H2 production and microbial diversity by anaerobic sludge treated chloroform with different concentrations were investigated in this work. Taking into account the cumulative H2 production, H2 percentage, maximum H2 production rate, H2 yield and substrate utilization efficiency together, 0.050% of CHCl3 concentration was found to be appropriate for enhancing anaerobic H2 production. The repeated batch tests revealed a good stability of H2 production with the inocula treated by chloroform at different concentrations. Denaturing gradient gel electrophoresis (DGGE) analysis demonstrates that CHCl3 showed desirable selective inhibition on methanogens, rather than H2-producing bacteria. The favorable microbial communities for fermentative H2 production by anaerobic sludge were uncultured bacterium clones nbt176c01, Janthinobacterium sp.7, Megasphaera paucivorans strain VTT E-03234, Clostridium cellulosi strain D3 and Clostridium sp. HPB-4.

AB - The stability of biological H2 production and microbial diversity by anaerobic sludge treated chloroform with different concentrations were investigated in this work. Taking into account the cumulative H2 production, H2 percentage, maximum H2 production rate, H2 yield and substrate utilization efficiency together, 0.050% of CHCl3 concentration was found to be appropriate for enhancing anaerobic H2 production. The repeated batch tests revealed a good stability of H2 production with the inocula treated by chloroform at different concentrations. Denaturing gradient gel electrophoresis (DGGE) analysis demonstrates that CHCl3 showed desirable selective inhibition on methanogens, rather than H2-producing bacteria. The favorable microbial communities for fermentative H2 production by anaerobic sludge were uncultured bacterium clones nbt176c01, Janthinobacterium sp.7, Megasphaera paucivorans strain VTT E-03234, Clostridium cellulosi strain D3 and Clostridium sp. HPB-4.

KW - Anaerobic H production

KW - CHCl treatment

KW - PCR-DGGE

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Evaluation of the stability of hydrogen production and microbial diversity by anaerobic sludge with chloroform treatment

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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