Evaluation of the safety of blood products with respect to human immunodeficiency virus infection by using an HTLV-I-infected cell line (SKT-1B)

Atsushi Koito, Kenji Shirono, Hiroyuki Suto, Shuzo Matsushita, Toshio Hattori, Kiyoshi Takatsuki

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

Human immunodeficiency virus (HIV), the etiologic agent of acquired immunodeficiency syndrome (AIDS), was rapidly cytopathic to SKT-1B, a cell line established from a patient with adult T cell leukemia, in vitro. This cytopathic effect was preceded by the expression of HIV antigen, defined with a monoclonal antibody (mAb) specific for the core protein (p24) of HIV. SKT-1B is highly susceptible to HIV as compared with MT-2 and H9 cells. HIV is known to be trasmitted via blood products, and thus we examined whether or not currently used procedures for manufacturing blood products are safe by using SKT-1B. Lyophilized HIV was heated at 65° for time periods in the range of 10 min to 48 hr, and the infectivity was examined. The results showed that heating at 65° for less than 2 hr,was not sufficient to inactivate HIV, but the virus heated for 48 hr had no effect on SKT-1B. In addition, HIV completely lost its infectivity on sulfonation, which is commonly used to avoid anaphylactic shock on intravenous infusion of human immunoglobulins. These findings indicate that blood products manufactured by currently used procedures are probably safe with respect to HIV infection.

Original languageEnglish
Pages (from-to)365-371
Number of pages7
JournalJapanese Journal of Cancer Research
Volume78
Issue number4
Publication statusPublished - 1987 Apr

Keywords

  • Adult T-cell leukemia
  • AIDS
  • HILV-I
  • Human immunodeficiency virus

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Fingerprint Dive into the research topics of 'Evaluation of the safety of blood products with respect to human immunodeficiency virus infection by using an HTLV-I-infected cell line (SKT-1B)'. Together they form a unique fingerprint.

Cite this