Evaluation of the enzyme activity of protozoan protein kinases by using an in vitro kinase assay

Kentaro Kato

doi:10.1016/j.parint.2016.07.006

Evaluation of the enzyme activity of protozoan protein kinases by using an in vitro kinase assay

Research output: Contribution to journal › Article › peer-review

Abstract

The life cycles of parasites are more complicated than those of other biological species. Protein kinases (PKs) encoded by parasites are the main triggers of life stage conversions. Phosphorylation by cellular PKs regulates important cellular processes, and the protozoan genome contains many PKs. Some PK inhibitors inhibit specific parasite life cycle event. In this report, I present a practical approach to expressing and purifying protozoan PKs by using a wheat germ cell-free protein synthesis system and I assess the phosphorylation activities of protozoan PKs by using an in vitro kinase assay.

Original language	English
Pages (from-to)	510-513
Number of pages	4
Journal	Parasitology International
Volume	65
Issue number	5
DOIs	https://doi.org/10.1016/j.parint.2016.07.006
Publication status	Published - 2016 Oct 1
Externally published	Yes

Keywords

In vitro kinase assay
Plasmodium falciparum
Protozoan protein kinases

ASJC Scopus subject areas

Parasitology
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.parint.2016.07.006

Cite this

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abstract = "The life cycles of parasites are more complicated than those of other biological species. Protein kinases (PKs) encoded by parasites are the main triggers of life stage conversions. Phosphorylation by cellular PKs regulates important cellular processes, and the protozoan genome contains many PKs. Some PK inhibitors inhibit specific parasite life cycle event. In this report, I present a practical approach to expressing and purifying protozoan PKs by using a wheat germ cell-free protein synthesis system and I assess the phosphorylation activities of protozoan PKs by using an in vitro kinase assay.",

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AU - Kato, Kentaro

N1 - Funding Information: This study was supported by grants-in-aid for Young Scientists from the Ministry of Education, Culture, Science, Sports, and Technology (MEXT) of Japan and by the Bio-oriented Technology Research Advancement Institution (BRAIN) . Publisher Copyright: © 2016 Elsevier Ireland Ltd

PY - 2016/10/1

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N2 - The life cycles of parasites are more complicated than those of other biological species. Protein kinases (PKs) encoded by parasites are the main triggers of life stage conversions. Phosphorylation by cellular PKs regulates important cellular processes, and the protozoan genome contains many PKs. Some PK inhibitors inhibit specific parasite life cycle event. In this report, I present a practical approach to expressing and purifying protozoan PKs by using a wheat germ cell-free protein synthesis system and I assess the phosphorylation activities of protozoan PKs by using an in vitro kinase assay.

AB - The life cycles of parasites are more complicated than those of other biological species. Protein kinases (PKs) encoded by parasites are the main triggers of life stage conversions. Phosphorylation by cellular PKs regulates important cellular processes, and the protozoan genome contains many PKs. Some PK inhibitors inhibit specific parasite life cycle event. In this report, I present a practical approach to expressing and purifying protozoan PKs by using a wheat germ cell-free protein synthesis system and I assess the phosphorylation activities of protozoan PKs by using an in vitro kinase assay.

KW - In vitro kinase assay

KW - Plasmodium falciparum

KW - Protozoan protein kinases

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Evaluation of the enzyme activity of protozoan protein kinases by using an in vitro kinase assay

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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