Evaluation of the binding of iron(II) to humic substances derived from a compost sample by a colorimetric method using ferrozine

Mitsuo Yamamoto, Atsushi Nishida, Keishi Otsuka, Takeshi Komai, Masami Fukushima

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

In evaluating the quality of composts that include humic substances (HSs), the binding of iron(II) (conditional binding constants and binding capacities) are an important criteria. To determine the binding ability, it is necessary to determine the concentrations of free Fe2+ and Fe(II)-HS complex species present. In the present study, we describe a colorimetric method for determining free Fe2+ species using ferrozine in an aqueous solution of Fe2+ and HS. It was found that the colored species in the Fe2+ and HS mixture represented only free Fe2+ species. Two water soluble fractions of HS, humic (HA) and fulvic (FA) acids, were isolated from a compost sample, and the conditional binding constants and binding capacities were estimated by colorimetric titration. Differences in the binding of Fe(II) to HA and FA are discussed, based on their structural features, which were characterized by acidic functional group analysis, elemental analysis and solid-state CP-MAS 13C NMR spectral data.

Original languageEnglish
Pages (from-to)4456-4460
Number of pages5
JournalBioresource Technology
Volume101
Issue number12
DOIs
Publication statusPublished - 2010 Jun 1
Externally publishedYes

Keywords

  • Binding capacity
  • Conditional binding constant
  • Ferrozine
  • Humic substances
  • Iron(II)

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

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