Abstract
Background: Cilostazol, a phosphodiesterase 3 inhibitor, is an antiplatelet drug that is widely used for preventing cardiovascular events, although, to date, there are few methods for evaluating its effects. Methods and Results: Blood samples were taken at baseline and at 3 and 12 h in 10 healthy male subjects after 100mg cilostazol intake. Each sample was examined by Western blot for phosphorylation levels of vasodilator-stimulated phosphoprotein (VASP), an abundant cAMP-dependent kinase substrate in platelets, and by the optical aggregometer for ADP- and collagen-induced aggregation, before and after 8 nmol/L prostaglandin E1 (PGE1) treatment. Cilostazol intake did not affect VASP phosphorylation levels or the maximal aggregation rates without PGE1 treatment. However, cilostazol intake apparently enhanced PGE1-induced VASP phosphorylation and PGE 1-mediated reduction of ADP- and collagen-induced maximal aggregation rates. Levels of VASP phosphorylated at Ser157 were correlated and the maximal aggregation rates induced by ADP were inversely correlated with cilostazol concentrations in the plasma. Conclusion: The antiplatelet effects of cilostazol intake could be evaluated by measuring VASP phosphorylation levels and maximal aggregation rates in platelets by ex vivo treatment with a low concentration of PGE1.
Original language | English |
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Pages (from-to) | 1844-1851 |
Number of pages | 8 |
Journal | Circulation Journal |
Volume | 72 |
Issue number | 11 |
DOIs | |
Publication status | Published - 2008 |
Externally published | Yes |
Keywords
- Antiplatelet
- Cilostazol
- Platelets
- Prostaglandins
- Signal transduction
ASJC Scopus subject areas
- Cardiology and Cardiovascular Medicine