Evaluation of the amount of the manganese entry in the neurons for activity-induced manganese-enhanced MRI

Satomi Kikuta, Yuchio Yanagawa, Noriyasu Homma, Makoto Osanai

Research output: Contribution to journalArticlepeer-review

Abstract

The activity-induced manganese-enhanced MRI (AIM-MRI) is one of the candidate methods for measuring the neuronal activity noninvasively in vivo. This method has the potential to measure the history of the neuronal activity, since the Mn2+ can enter the active neuron through voltage-dependent Ca2+ channels. However, it has not been clear that the relation between the neuronal activity and the accumulation of Mn2+ in the cell. At first, we confirmed that the longitudinal relaxation time of H+ (T1) measured by means of MRI apparatus was related to the [Mn2+] using the pseudo intracellular phantoms with various concentration of Mn2+. The inverse of T1(1/T1) was proportional to [Mn2+], thus [Mn2+] can be measured by MRI quantitatively. Next, we investigated the relation between the neuronal activity and the accumulation of Mn2+ in the striatal GABAergic neurons using the method of the Mn2+ quenching of Fura-2 LR, a family of fura fluorescent Ca2+ indicators. The amount of the Ca2+ influx was correlated to the neuronal activity evoked by tetanic stimulations with various frequencies. In the same slice preparation, the amounts of the fluorescence quench induced by the tetanic stimulations under the condition of 50 μM MnCl2 administration were recorded. As a result, the amount of the quench was proportional to the amount of the Ca2+ influx. These results suggested that a cell, which has higher activity, accumulated larger amount of Mn2+ in the neuron. Thus, our results supported that AIM-MRI can measure the history of the neuronal activities in the whole brain noninvasively in vivo.

Original languageEnglish
Pages (from-to)280-284
Number of pages5
JournalIEEJ Transactions on Electronics, Information and Systems
Volume135
Issue number3
DOIs
Publication statusPublished - 2015 Mar 1

Keywords

  • Calcium imaging
  • MRI
  • Manganese

ASJC Scopus subject areas

  • Electrical and Electronic Engineering

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