@article{665910c8e4b243929d26c1be32f0909a,
title = "Evaluation of squalene oxidation mechanisms in human skin surface lipids and shark liver oil supplements",
abstract = "Squalene is a terpenoid found in human skin surface lipids (SSLs) and foods that possesses beneficial properties. However, since oxidation of squalene causes various complications, it is necessary to identify the mechanisms by which squalene is oxidized. In this study, we aimed to determine the oxidation mechanisms of squalene in SSLs and shark liver oil (SLO) supplements by the analysis of squalene monohydroperoxide (SQOOH) isomers, on the basis of our previous finding that different oxidation mechanisms yield different SQOOH isomers. Liquid chromatography–tandem mass spectrometry analysis of SQOOH isomers revealed that squalene in human SSLs was oxidized by singlet oxygen oxidation, whereas squalene in SLO was oxidized mainly by free radicals. As a result, we have presented the first evidence suggesting that the analysis of SQOOH isomers enables estimation of oxidation mechanisms. Estimating oxidation mechanisms by analyzing SQOOH isomers may provide a foundation for the prevention of skin diseases and food deterioration via regulation of squalene oxidation.",
keywords = "LC−MS/MS, lipid oxidation mechanisms, shark liver oil, skin surface lipids, squalene monohydroperoxide",
author = "Naoki Shimizu and Junya Ito and Shunji Kato and Takahiro Eitsuka and Tetsuo Saito and Hiroshi Nishida and Teruo Miyazawa and Kiyotaka Nakagawa",
note = "Funding Information: This work was supported in part by the Japan Society for the Promotion of Science (JSPS) through JSPS Core-to-Core Program (Advanced Research Networks) entitled “Establishment of international agricultural immunology research-core for a quantum improvement in food safety” and JSPS KAKENHI grant number JP19J21890. H.N. is a representative director and T.S. is an employee of a commercial company Nissei Marine Industrial Co., Ltd. The company provided support in the form of remuneration for H.N. and T.S., but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. Funding Information: This work was supported in part by the Japan Society for the Promotion of Science (JSPS) through JSPS Core-to-Core Program (Advanced Research Networks) entitled ?Establishment of international agricultural immunology research-core for a quantum improvement in food safety? and JSPS KAKENHI grant number JP19J21890. H.N. is a representative director and T.S. is an employee of a commercial company Nissei Marine Industrial Co., Ltd. The company provided support in the form of remuneration for H.N. and T.S., but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. Publisher Copyright: {\textcopyright} 2019 New York Academy of Sciences.",
year = "2019",
month = dec,
day = "1",
doi = "10.1111/nyas.14219",
language = "English",
volume = "1457",
pages = "158--165",
journal = "Annals of the New York Academy of Sciences",
issn = "0077-8923",
publisher = "Wiley-Blackwell",
number = "1",
}
