Evaluation of retinal degeneration in P27KIP1 null mouse

Yumi Tokita-Ishikawa, Ryosuke Wakusawa, Toshiaki Abe

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

Purpose: P27kip1 is well-known as a cell cycle inhibitor and also plays an important role for cell differentiation. We hypothesized that if we caused retinal degeneration in a p27(-/-) mouse, then the appropriate method of restoration may be different from that of wild mice and therefore suggest a therapeutic methodology for retinal regeneration. Methods: Histological and electrophysiological (ERG) examination was performed on p27(-/-) mice retina. We injected N-methy-N-nitrosourea (MNU) to induce retinal degeneration. BrdU was used to identify the dividing cells in the retina. Results: Thicker retina were observed in the p27(-/-) mice when compared to those of the p27(-/+) mice or wild type mice. Almost all retinal layers were thick and optic nerves were also enlarged. A statistically significant decrease of a and b waves amplitudes of ERG was observed in p27(-/-) mice when compared to those of the other mice. BrdU and nestin positive cells were present at the outer nuclear layer with no difference between p27(-/-) and wild type mice after MNU injection. Conclusion: p27(-/-) mice showed thicker retina and less retinal function than those of other mice. The MNU-induced retinal degeneration in p27(-/-) mice closely resembled the reaction of the other mice with no retinal regeneration observed in our experimental condition.

Original languageEnglish
Title of host publicationRetinal Degenerative Diseases
Subtitle of host publicationLaboratory and Therapeutic Investigations
EditorsRobert Anderson, Nawajes Mandal, Joe Hollyfield, Matthew LaVail
Pages467-471
Number of pages5
DOIs
Publication statusPublished - 2010 Dec 1

Publication series

NameAdvances in Experimental Medicine and Biology
Volume664
ISSN (Print)0065-2598

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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  • Cite this

    Tokita-Ishikawa, Y., Wakusawa, R., & Abe, T. (2010). Evaluation of retinal degeneration in P27KIP1 null mouse. In R. Anderson, N. Mandal, J. Hollyfield, & M. LaVail (Eds.), Retinal Degenerative Diseases: Laboratory and Therapeutic Investigations (pp. 467-471). (Advances in Experimental Medicine and Biology; Vol. 664). https://doi.org/10.1007/978-1-4419-1399-9_53