On-column dissociation rate constant (kd) of the Ni(II) complex with pyridine-2-aldehydebenzoylhydrazone (PAB) has been determined using the high performance liquid chromatographic kinetic analysis method to estimate the effect of the properties of the stationary phase on the oncolumn dissociation reaction characteristics of Ni(II)-PAB complex. In the case of using the column with endcapped packing material, smaller kd was obtained as compared with that in the case of using the column with non-endcapped one. The dissociation rate constants of the Ni(II)-PAB complex in the stationary phase and those of in the mobile phase (kd,s, kd,m) was estimated from the values of kd. The value of kd,s reduced with the addition of tetrabutylammonium ion to the eluent as the blocking agent of the silanol groups. It was thus suggested that the on-column dissociation reaction of the Ni(II)-PAB complex was accelerated by the silanol groups on the surface of the silica support. It is cleared that the dissociation reaction of the Ni(II)-PAB complex in the stationary phase dominates the total on-column dissociation reaction of the complex and the properties of the stationary phase are the important factor to determine the on-column dissociation reaction characteristics of the metal complexes.
- Dissociation kinetic analysis
- Dissociation rate constant
- High performance liquid chromatography
- Metal complex
- Silanol groups
ASJC Scopus subject areas
- Analytical Chemistry