Evaluation of mitochondrial respiratory function in rat cardiomyocytes under different glucose conditions, using an extracellular flux analysis method

Hirokazu Tsubone, Tatsushi Mutoh

Research output: Contribution to journalLetterpeer-review

Abstract

We studied the effect of glucose supply on mitochondrial respiratory function (MRF) of neonatal rat cardiomyocytes, using a novel extracellular flux analysis. Fundamental respiratory parameters regarding oxygen consumption rate in mitochondria showed glucose concentration-dependent changes, where a significant increase or decrease in these parameters was observed to be associated with glucose concentrations ranging between 10% and 1000% of the concentration used in standard medium (3151 mg/L), respectively. By contrast, the extracellular acidification rate, a parameter of anaerobic activity, was shown to decrease under low-glucose conditions, whereas it increased after inhibiting complex V (ATP synthase) under the same glucose conditions. These findings provide a beneficial basis for various experimental studies on mitochondrial metabolism in cardiomyocytes.

Original languageEnglish
Pages (from-to)1330-1333
Number of pages4
JournalClinical and Experimental Pharmacology and Physiology
Volume45
Issue number12
DOIs
Publication statusPublished - 2018 Dec

Keywords

  • cardiomyocyte
  • glucose
  • mitochondria
  • oxygen consumption
  • respiration

ASJC Scopus subject areas

  • Physiology
  • Pharmacology
  • Physiology (medical)

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