Evaluation of IFCC standardization method in alkaline phosphatase (ALP) measurement

Yoshie Goto, Katsuyuki Sasaki, Kazushi Kashio, Yurina Yokota, Takuya Takeda, Atsuko Hiraizumi, Shin Ichi Fujimaki, Tohru Fujiwara

Research output: Contribution to journalArticlepeer-review

Abstract

Japan Society of Clinical Chemistry (JSCC) has decided that JSCC-based reference method to measure alkaline phosphatase (ALP) will be changed to the International Federation of Clinical Chemistry and Laboratory Medicine (IFCC)-based method from April 2020, allowing one-year transitional period. By analyzing ALP value based on both JSCC and IFCC method, we evaluated their correlation and the potential discrepancies caused by differential reactivity of specific isozymes, such as placental and intestinal isozymes. When the placental and the intestinal isozymes were excluded for the analysis, we confirmed the high validity of the conversion factor (2.84 times) from IFCC to JSCC method. In this study, we investigated the relationship between the emergence of placental isozyme and gestational weeks. For intestinal isozyme, we evaluated the effect of food intake (outpatients versus inpatient) as well as blood type. These results would provide useful information for all medical staffs.

Original languageEnglish
Pages (from-to)199-205
Number of pages7
JournalJapanese Journal of Clinical Chemistry
Volume49
Issue number3
Publication statusPublished - 2020

Keywords

  • Alkaline phosphatase(ALP)
  • Conversion factor
  • IFCC reference method
  • Intestinal alkaline phosphatase
  • Placental alkaline phosphatase

ASJC Scopus subject areas

  • Clinical Biochemistry

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