Evaluation of damage in DNA molecules caused by very-low-frequency magnetic fields using bacterial cells

Akira Haga, Yoshiaki Kumagai, Hidetoshi Matsuki, Ginro Endo, Akira Igarashi, Koichiro Kobayashi

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The effect of intermediate frequency magnetic fields or, very-low-frequency magnetic fields (VLFMF) on living biological cells was investigated using a highly sensitive mutagenesis assay method. A bacterial gene expression system for mutation repair (umu system) was used for the sensitive evaluation of damage in DNA molecules. Salmonella typhimurium TA1535 (pSK1002) were exposed to VLFMF (20 kHz and 600 μT) in a specially designed magnetic field loading chamber. The experiment results showed the possibility of applying the umu assay for sensitive and effective evaluation of damage in DNA molecules. No effects from exposure to 20 kHz and 600 μT magnetic fields in terms of damage in DNA molecules were observed.

Original languageEnglish
Pages (from-to)3249-3255
Number of pages7
JournalIEICE Transactions on Communications
VolumeE88-B
Issue number8
DOIs
Publication statusPublished - 2005 Aug

Keywords

  • LacZ, Y, β-galactosidase
  • Mutation repairing gene
  • Salmonella typhimurium TA1535 (pSK1002)
  • Umu operon
  • Very-low-frequency magnetic field (VLFMF)

ASJC Scopus subject areas

  • Software
  • Computer Networks and Communications
  • Electrical and Electronic Engineering

Fingerprint Dive into the research topics of 'Evaluation of damage in DNA molecules caused by very-low-frequency magnetic fields using bacterial cells'. Together they form a unique fingerprint.

  • Cite this