TY - JOUR
T1 - Evaluation of acoustic properties of the live human smooth-muscle cell using scanning acoustic microscopy
AU - Kinoshita, Aki
AU - Senda, Shoichi
AU - Mizushige, Katsufumi
AU - Masugata, Hisashi
AU - Sakamoto, Seiji
AU - Kiyomoto, Hideyasu
AU - Matsuo, Hirohide
N1 - Funding Information:
We express our thanks to Prof. H. Nakazawa, M.D., of the Department of Physiology, Tokai University, and K. Katakura, PhD., of Hitachi Co., Ltd. for helpful discussions and encouragement. This work was supported in part by a Grant-in-Aid for Scientific Research (B) No. 08458289 (to S. S.) from the Ministry of Education, Science, Sports and Culture of Japan.
PY - 1998/12
Y1 - 1998/12
N2 - This study was performed to measure the acoustic propagation speed in live human aortic smooth-muscle cells (HASMC), using scanning acoustic microscopy (SAM) and a novel measurement theory that permits the measurement of the acoustic propagation speed in biological samples of unknown thickness. C-mode and X-Z-mode images of HASMC under three different conditions: growing (G); differential (D); and on hypotonic loading (H), were acquired using 100- MHz, 450-MHz and 600-MHz ultrasound. The images exhibit features related to the cell surface curvature and intracellular structure. The theory supporting the methodology is derived in this article and makes use of the interference fringes within the focusing lens of the high-frequency transducer. The propagation speed in the cells was calculated from the location of the interference fringe on the C-mode images and the fringe shift on the X-Y- mode images with 450-MHz ultrasound. The propagation speed in D (1624 ± 16 m/s) was significantly higher than those in G (1571 ± 14 m/s, p < 0.05) and H (1585 ± 8 m/s, p < 0.05). Scanning acoustic microscope measurements, along with the described theory, are useful for studying the acoustic properties of live cells ex vivo and have applications in both pathophysiology and biomechanics.
AB - This study was performed to measure the acoustic propagation speed in live human aortic smooth-muscle cells (HASMC), using scanning acoustic microscopy (SAM) and a novel measurement theory that permits the measurement of the acoustic propagation speed in biological samples of unknown thickness. C-mode and X-Z-mode images of HASMC under three different conditions: growing (G); differential (D); and on hypotonic loading (H), were acquired using 100- MHz, 450-MHz and 600-MHz ultrasound. The images exhibit features related to the cell surface curvature and intracellular structure. The theory supporting the methodology is derived in this article and makes use of the interference fringes within the focusing lens of the high-frequency transducer. The propagation speed in the cells was calculated from the location of the interference fringe on the C-mode images and the fringe shift on the X-Y- mode images with 450-MHz ultrasound. The propagation speed in D (1624 ± 16 m/s) was significantly higher than those in G (1571 ± 14 m/s, p < 0.05) and H (1585 ± 8 m/s, p < 0.05). Scanning acoustic microscope measurements, along with the described theory, are useful for studying the acoustic properties of live cells ex vivo and have applications in both pathophysiology and biomechanics.
KW - Acoustic propagation speed
KW - Acoustic property
KW - Biomechanics
KW - Live cell
KW - Pathophysiology
KW - Scanning acoustic microscopy
KW - Smooth muscle cell
KW - Tissue characterization
KW - Ultrasound
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U2 - 10.1016/S0301-5629(98)00121-5
DO - 10.1016/S0301-5629(98)00121-5
M3 - Article
C2 - 10385962
AN - SCOPUS:0032428373
VL - 24
SP - 1397
EP - 1405
JO - Ultrasound in Medicine and Biology
JF - Ultrasound in Medicine and Biology
SN - 0301-5629
IS - 9
ER -