Evaluation of a low-temperature steam and formaldehyde sterilizer

K. Kanemitsu, H. Kunishima, T. Imasaka, S. Ishikawa, H. Harigae, S. Yamato, Y. Hirayama, M. Kaku

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)


We evaluated a low-temperature steam and formaldehyde (LTSF) sterilizer based on the draft European Standard prEN 14180. Microbiological tests were conducted on small and full loads using process challenge devices in five programs (P1-P5). With small loads all tests showed no growth of Bacillus stearothermophilus (ATCC7953) spores. However, positive cultures were observed with full-load tests using P5 (sterilization temperature, 50°C). Our data indicated that the load influenced the efficacy of the LTSF sterilizer. Desorption tests were conducted to determine residual formaldehyde in indicator strips. The mean concentrations of formaldehyde in P1-P5 were 31.9, 56.3, 54.9, 82.2 and 180.6 μg, respectively, which are below the limits allowed by the draft Standard. Our results indicate that the LTSF sterilizer is useful for sterilization because of its excellent efficacy, short handling time, and safety.

Original languageEnglish
Pages (from-to)47-52
Number of pages6
JournalJournal of Hospital Infection
Issue number1
Publication statusPublished - 2003 Sep


  • Bacillus stearothermophilus
  • Draft European Standard prEN 14180
  • Low-temperature steam and formaldehyde sterilizer

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases


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