Evaluating Young's Modulus of Single Yeast Cells Based on Compression Using an Atomic Force Microscope with a Flat Tip

DI Chang, Takahiro Hirate, Chihiro Uehara, Hisataka Maruyama, Nobuyuki Uozumi, Fumihito Arai

Research output: Contribution to journalArticlepeer-review

Abstract

In this research, atomic force microscopy (AFM) with a flat tip cantilever is utilized to measure Young's modulus of a whole yeast cell (Saccharomyces cerevisiae BY4741). The results acquired from AFM are similar to those obtained using a microfluidic chip compression system. The mechanical properties of single yeast cells are important parameters which can be examined using AFM. Conventional studies apply AFM with a sharp cantilever tip to indent the cell and measure the force-indentation curve, from which Young's modulus can be calculated. However, sharp tips introduce problems because the shape variation can lead to a different result and cannot represent the stiffness of the whole cell. It can lead to a lack of broader meaning when evaluating Young's modulus of yeast cells. In this report, we confirm the differences in results obtained when measuring the compression of a poly(dimethylsiloxane) bead using a commercial sharp tip versus a unique flat tip. The flat tip effectively avoids tip-derived errors, so we use this method to compress whole yeast cells and generate a force-deformation curve. We believe our proposed method is effective for evaluating Young's modulus of whole yeast cells.

Original languageEnglish
Pages (from-to)392-399
Number of pages8
JournalMicroscopy and Microanalysis
Volume27
Issue number2
DOIs
Publication statusPublished - 2021 Apr

Keywords

  • Young's modulus
  • atomic force microscopy
  • compression
  • microfluidic chip
  • yeast cell

ASJC Scopus subject areas

  • Instrumentation

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