Eucalyptus has functional equivalents of the Arabidopsis AP1 gene

Junko Kyozuka; R. Harcourt; W. J. Peacock; E. S. Dennis

doi:10.1023/A:1005885808652

Eucalyptus has functional equivalents of the Arabidopsis AP1 gene

Junko Kyozuka, R. Harcourt, W. J. Peacock, E. S. Dennis

Research output: Contribution to journal › Article › peer-review

79 Citations (Scopus)

Abstract

Two Eucalyptus homologues of the Arabidopsis floral homeotic gene AP1 (EAP1 and EAP2) show 60-65% homology to AP1. EAP1 and EAP2 are expressed predominantly in flower buds. EAP2 produces two different polypeptides arising from differential splicing at an intron, the shorter EAP2 protein diverging from the longer sequence after amino acid 197 and having a translation stop after residue 206. This truncated protein includes both MADS- and K-box amino acid sequences. Ectopic expression of the EAP1 or either of the two EAP2 polypeptides in Arabidopsis driven by the 35S promoter produces effects similar to the corresponding AP1 construct, causing plants to flower earlier, have shorter bolts and resemble the terminal flower mutant (tfl).

Original language	English
Pages (from-to)	573-584
Number of pages	12
Journal	Plant Molecular Biology
Volume	35
Issue number	5
DOIs	https://doi.org/10.1023/A:1005885808652
Publication status	Published - 1997 Nov
Externally published	Yes

Keywords

Arabidopsis thaliana
Eucalyptus
Floral homeotic gene

ASJC Scopus subject areas

Genetics
Agronomy and Crop Science
Plant Science

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title = "Eucalyptus has functional equivalents of the Arabidopsis AP1 gene",

abstract = "Two Eucalyptus homologues of the Arabidopsis floral homeotic gene AP1 (EAP1 and EAP2) show 60-65% homology to AP1. EAP1 and EAP2 are expressed predominantly in flower buds. EAP2 produces two different polypeptides arising from differential splicing at an intron, the shorter EAP2 protein diverging from the longer sequence after amino acid 197 and having a translation stop after residue 206. This truncated protein includes both MADS- and K-box amino acid sequences. Ectopic expression of the EAP1 or either of the two EAP2 polypeptides in Arabidopsis driven by the 35S promoter produces effects similar to the corresponding AP1 construct, causing plants to flower earlier, have shorter bolts and resemble the terminal flower mutant (tfl).",

keywords = "Arabidopsis thaliana, Eucalyptus, Floral homeotic gene",

author = "Junko Kyozuka and R. Harcourt and Peacock, {W. J.} and Dennis, {E. S.}",

note = "Funding Information: We thank Xiaomei Zhu and Janice Norman for excellent technical help. We also thank Martin Yanofsky for providing Arabidopsis AP1 cDNA clones; Stuart Craig for scanning electron microscopy; Mike Mon-cur and Stuart Craig for the photography of the Eucalyptus buds; Rudy Dolferus for photography; and Abed Chaudhury, Greg Gocal, Danny Llewellyn and Simon Southerton for discussion and critical reading of the manuscript. This work was supported by a grant from the Australian Department of Industry, Science and Technology and by North Eucalypt Technologies, Australian Newsprint Mills, Australian Paper Mills and Kimberly Clark.",

year = "1997",

month = nov,

doi = "10.1023/A:1005885808652",

language = "English",

volume = "35",

pages = "573--584",

journal = "Plant Molecular Biology",

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AU - Kyozuka, Junko

AU - Harcourt, R.

AU - Peacock, W. J.

AU - Dennis, E. S.

N1 - Funding Information: We thank Xiaomei Zhu and Janice Norman for excellent technical help. We also thank Martin Yanofsky for providing Arabidopsis AP1 cDNA clones; Stuart Craig for scanning electron microscopy; Mike Mon-cur and Stuart Craig for the photography of the Eucalyptus buds; Rudy Dolferus for photography; and Abed Chaudhury, Greg Gocal, Danny Llewellyn and Simon Southerton for discussion and critical reading of the manuscript. This work was supported by a grant from the Australian Department of Industry, Science and Technology and by North Eucalypt Technologies, Australian Newsprint Mills, Australian Paper Mills and Kimberly Clark.

PY - 1997/11

Y1 - 1997/11

N2 - Two Eucalyptus homologues of the Arabidopsis floral homeotic gene AP1 (EAP1 and EAP2) show 60-65% homology to AP1. EAP1 and EAP2 are expressed predominantly in flower buds. EAP2 produces two different polypeptides arising from differential splicing at an intron, the shorter EAP2 protein diverging from the longer sequence after amino acid 197 and having a translation stop after residue 206. This truncated protein includes both MADS- and K-box amino acid sequences. Ectopic expression of the EAP1 or either of the two EAP2 polypeptides in Arabidopsis driven by the 35S promoter produces effects similar to the corresponding AP1 construct, causing plants to flower earlier, have shorter bolts and resemble the terminal flower mutant (tfl).

AB - Two Eucalyptus homologues of the Arabidopsis floral homeotic gene AP1 (EAP1 and EAP2) show 60-65% homology to AP1. EAP1 and EAP2 are expressed predominantly in flower buds. EAP2 produces two different polypeptides arising from differential splicing at an intron, the shorter EAP2 protein diverging from the longer sequence after amino acid 197 and having a translation stop after residue 206. This truncated protein includes both MADS- and K-box amino acid sequences. Ectopic expression of the EAP1 or either of the two EAP2 polypeptides in Arabidopsis driven by the 35S promoter produces effects similar to the corresponding AP1 construct, causing plants to flower earlier, have shorter bolts and resemble the terminal flower mutant (tfl).

KW - Arabidopsis thaliana

KW - Eucalyptus

KW - Floral homeotic gene

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Eucalyptus has functional equivalents of the Arabidopsis AP1 gene

Abstract

Keywords

ASJC Scopus subject areas

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